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Applied Microbiology and Biotechnology

, Volume 26, Issue 4, pp 318–322 | Cite as

Purification of extracellular alkaline phosphatase released by Escherichia coli excretory mutants

  • Danielle Atlan
  • Raymond Portalier
Biotechnology

Summary

Alkaline phosphatase (APase) is the major protein released into the extracellular medium by strain 706, a periplasmic-excretory (lky) mutant of Escherichia coli K12. We developed a rapid three step procedure for APase purification from culture supernatants of lky mutants. Two ultrafiltration stages and an heat treatment were sufficient to obtain a 99% pure enzyme preparation. Batch culture conditions of strain 706 in a 15 l fermentor leading to an extracellular APase yield of 1250 U/ml were determined.

Keywords

Enzyme Escherichia Coli Purification Heat Treatment Alkaline Phosphatase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

APase

E. coli alkaline phosphatase

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Danielle Atlan
    • 1
  • Raymond Portalier
    • 1
  1. 1.Atelier de Biotechnologie Enzymatique et Microbienne (UM 24)Université Claude BernardVilleurbanne CedexFrance

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