Urological Research

, Volume 17, Issue 3, pp 177–180 | Cite as

The influence of zinc and citrate on urease-induced urine crystallisation

  • H. Hedelin
  • L. Grenabo
  • J. Hugosson
  • S. Pettersson
Original Articles
Accepted:

Summary

Zinc reduced and citrate promoted the urease-induced pH increase in synthetic urine. Secondary to this, the precipitation of magnesium ammonium phosphate and calcium phosphate was influenced. Independent of this pH-related effect, zinc also increased the precipitation of magnesium ammonium phosphate and decreased the calcium phosphate precipitation. These observations were not totally reproducable in human urine.

Key words

Urease-induced crystallisation Citrate Zinc 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Burr RG, Nuseibeth, Abiaka CD (1985) Biochemical studies in paraplegic renal stone patients. Br J Urol 57:275–278Google Scholar
  2. 2.
    Graef V, Schmidtmann, Jarrar K (1985) On the preservation of urines for the determination of citrate. In: Schwille PO (ed) Urolithiasis and related clinical research. Plenum Press, New York, pp 689–692Google Scholar
  3. 3.
    Grenabo L, Hedelin H, Pettersson S (1986) The inhibitory effect of human urine on urease-induced crystallisation in vitro. J Urol 135:416–419Google Scholar
  4. 4.
    Griffith DP, Musher DM, Itin C (1976) Urease the primary cause of infection-induced urinary stones. Invest Urol 13:346–350Google Scholar
  5. 5.
    Hedelin H, Grenabo L, Pettersson S (1985) Urease-induced crystallisation in synthetic urine. Invest Urol 133:529–531Google Scholar
  6. 6.
    Hedelin H, Grenabo L, Pettersson S (1986) The effects of urease in undiluted human urine. J Urol 136:743–745Google Scholar
  7. 7.
    Hughes RB, Sidney AK, Stubbins SE (1969) Inhibition of urease by metal ions. Enzymologia 36:332–334Google Scholar
  8. 8.
    Meyer JL, Angino EE (1977) The role of trace metals in calcium urolithiasis. Invest Urol 14:347–350Google Scholar
  9. 9.
    Meyer JL, Thomas WC (1982) Trace metal-citric acid complexes as inhibitors of calcification and crystal growth. J Urol 128:1372–1376Google Scholar
  10. 10.
    Nicar MJ, Skurta C, Sakhall K, Pak C (1983) Low urinary citrate excretion in nephrolithiasis. Urology 21:8–14Google Scholar
  11. 11.
    Ogawa Y, Morozumi M, Tanaka T, Yamaguchi K (1986) Determination of urinary citrate by ion chromotography. J Urol 135:178–181Google Scholar
  12. 12.
    Pak C, Ruskin B, Diller E (1972) Enhancement of renal excretion of zinc by hydrochlorothiazide. Clin Chim Acta 39:511–517Google Scholar
  13. 13.
    Robertson WG, Nordin BEE (1976) Physico-chemical factors govering stone formation. In: William DI, Chisholm GD (eds) Scientific foundations of urology, vol 1. Heinemann Medical, London, p 234Google Scholar
  14. 14.
    Savory J, Wiggins JW, Heintges MG (1969) Measurements of calcium and magnesium in serum and urine by atomic absorption spectrometry. Am J Clin Pathol 51:720Google Scholar
  15. 15.
    Spencer H, Osis D, Kramer L, Norris C (1976) Intake, excretion and retention of zinc in man. In: Prasad AS, Oberleas D (eds) Trace elements in human health and disease, vol 1. Academic Press, New York, pp 345–359Google Scholar
  16. 16.
    Tompkins D, Toffaletti J (1982) Enzymic determination of citrate in serum and urine, with use of the Worthington “ultrafree” device. Clin Chem 28:192–195Google Scholar
  17. 17.
    Zilversmith OB, Davis AK (1950) Microdetermination of plasmaphospholipids by trichloroacetid acid precipitation. J Lab Clin Med 35:155–158Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • H. Hedelin
    • 1
  • L. Grenabo
    • 1
  • J. Hugosson
    • 1
  • S. Pettersson
    • 1
  1. 1.Department of Urology, Sahlgrenska HospitalUniversity of GöteborgGöteborgSweden

Personalised recommendations