Urological Research

, Volume 13, Issue 3, pp 143–148

The screening of more than 2,000 schoolgirls for bacteriuria using an automated fluorescence microscopy system

  • R. Manson
  • J. Scholefield
  • R. J. Johnston
  • R. Scott
Originals

Summary

After initial evaluation of a manual fluorescence microscopy system on a variety of urines the method was automated and subsequently tested in a population survey of urinary tract infection in schoolgirls. This automated Bactoscan system allowed a rapid analysis of urine samples and with the introduction of modifications to the staining protocol it correctly eliminated 91% of samples as being not significantly infected.

Key words

Bacteriuria Rapid detection Acridine Orange Fluorescence Bactoscan 

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References

  1. 1.
    Scholefield J, Manson R, Johnston RJ, Scott R (1985) The use of acridine orange staining and immage analysis to detect bacteriuria. Urol Res 13:141–142Google Scholar
  2. 2.
    Scott R, Scholefield J, Manson R, Johnston RJ (1985) A survey of bacteriuria in 2,234 schoolgirls in a Scottish new town. Urol Res 13:149–153Google Scholar
  3. 3.
    Spinell M (1982) Bakterienzählung mit dem Bactoscan-Gerät. Deutsche Milchwirtschaft 33:19–21Google Scholar
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    Nie MH, Hull CH, Jenkins J, Steinbrenner KS, Bent DH (eds) (1975) Statistical package for the social sciences. McGraw Hill CoGoogle Scholar
  5. 5.
    Pezzlo MT (1983) Automated methods for detection of bacteriuria. Am J Med 75:71–78Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • R. Manson
    • 1
  • J. Scholefield
    • 1
  • R. J. Johnston
    • 1
  • R. Scott
    • 2
  1. 1.Department of Bioscience Biotechnology, Food Science Division, Rapid Methods Microscopy UnitUniversity of StrathclydeGlasgow
  2. 2.Department of UrologyRoyal InfirmaryGlasgowUK

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