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Urological Research

, Volume 10, Issue 4, pp 191–194 | Cite as

Conductivometric determination of urinary oxalate with oxalate decarboxylase

  • M. Bishop
  • H. Freudiger
  • U. Largiadèr
  • J. D. Sallis
  • R. Felix
  • H. Fleisch
Originals

Summary

An enzymatic method for determination of urinary oxalate is described: the acidified urine samples are extracted with chloroform. This manipulation improves the blank values considerably. 1 ml of extracted urine is incubated with oxalate decarboxylase. The CO2 released from the medium is absorbed by Sr(OH)2. The change in conductivity measured in the Sr(OH)2 solution is linearly proportional to the oxalate concentration in urine and the method is specific for oxalate. The mean recovery is 93.2±2.5%. The coefficient of variation calculated from 28 determinations is 12.6%. The detection limit is 35 nmol. 1 ml of urine is usually sufficient for determination. The mean 24 h urine oxalate excretion of 11 healthy men and 16 women was 240±20 μmol.

Key words

Urine, Oxalate Conductivometry Oxalate decarboxylase 

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References

  1. 1.
    Bais R, Potezny N, Edwards JB, Rofe AM, Conyers RAJ (1980) Oxalate determination by immobilized oxalate oxidase in a continuous flow system. Anal Chem 52:508–511Google Scholar
  2. 2.
    Charransol G, Barthelemy CH, Desgrez P (1978) Rapid determination of urinary oxalic acid by gas-liquid chromatography without extraction. J Chromatogr 145:452–455Google Scholar
  3. 3.
    Chiriboga J (1966) Purification and properties of oxalic acid oxidase. Arch Biochem Biophys 116:516–523Google Scholar
  4. 4.
    Costello J, Hatch M, Bourke E (1976) An enzymatic method for the spectrophotometric determination of oxalic acid. J Lab Clin Med 87:903–908Google Scholar
  5. 5.
    Farrington CJ, Chalmers AH (1979) Gas-chromatographic estimation of urinary oxalate and its comparison with a colorimetric method. Clin Chem 25:1993–1996Google Scholar
  6. 6.
    Gelot MA, Lavoue G, Belleville F, Nabet P (1980) Determination of oxalates in plasma and urine using gas chromatography. Clin Chim Acta 106:279–285Google Scholar
  7. 7.
    Hallson PC, Rose GA (1974) A simplified and rapid enzymatic method for determination of urinary oxalate. Clin Chim Acta 55:29–39Google Scholar
  8. 8.
    Hodgkinson A, Williams A (1972) An improved colorimetric procedure for urine oxalate. Clin Chim Acta 36:127–132Google Scholar
  9. 9.
    Hodgkinson A (1981) Sampling errors in the determination of urine calcium and oxalate: solubility of calcium oxalate in HCl-urine mixtures. Clin Chim Acta 109:239–244Google Scholar
  10. 10.
    Kobos RK, Ramsey RA (1980) Enzyme electrode system for oxalate determination utilizing oxalate decarboxylase immobilized on a carbon dioxide sensor. Anal Chim Acta 121:111–118Google Scholar
  11. 11.
    Kohlbecker G, Richter L, Butz M (1979) Detemination of oxalate in urine using oxalate oxidase: Comparison with oxalate decarboxylase. J Clin Chem Chin Biochem 17:309–313Google Scholar
  12. 12.
    Laker MF, Hofmann AF, Meeuse BJD (1980) Spectrophotometric determination of urinary oxalate with oxalate oxidase prepared from moss. Clin Chem 26:827–830Google Scholar
  13. 13.
    Mayer WJ, Greenberg MS (1979) A comparison of differential pulse and D. C. amperometric detection modes for the liquid chromatographic determination of oxalic acid. J Chromatogr Sci 17:614–616Google Scholar
  14. 14.
    Mayer WJ, McCarthy JP, Greenberg MS (1979) The determination of oxalic acid in urine by high performance liquid chromatography with electrochemical detection. J Chromatogr Sci 17:656–660Google Scholar
  15. 15.
    Moye HA, Malagodi MH, Clarke DH, Miles CJ (1981) A rapid gas chromatographic procedure for the analysis of oxalate ion in urine. Clin Chim Acta 114:173–185Google Scholar
  16. 16.
    Park KY, Gregory J (1980) Gas-chromatographic determination of urinary oxalate. Clin Chem 26:1170–1172Google Scholar
  17. 17.
    Sachs L (1973) Angewandte Statistik, 4th edition. Springer Verlag, Berlin, Heidelberg, New York, p 160Google Scholar
  18. 18.
    Sallis JD, Lumley MF, Jordan JE (1977) An assay for oxalate based on the conductometric measurement of enzyme-liberated carbon dioxide. Biochem Med 18:371–377Google Scholar
  19. 19.
    Schmidt K, Hagmaier V, Bruchelt G, Rutishauser G (1980) Analytical isotachophoresis: a rapid and sensitive method for determination of urinary oxalate. Urol Res 8:177–180Google Scholar
  20. 20.
    Shimazono H, Hayaishi O (1957) Enzymatic decarboxylation of oxalic acid. J Biol Chem 227:151–159Google Scholar
  21. 21.
    Tocco DJ, Duncan AEW, Noll RM, Duggan DE (1979) An electron-capture gas chromatographic procedure for the estimation of oxalic acid in urine. Anal Biochem 94:470–476Google Scholar
  22. 22.
    Yriberri J, Posen S (1980) A semi-automatic enzymic method for estimating urinary oxalate. Clin Chem 26:881–884Google Scholar
  23. 23.
    Zarembski PM, Hodgkinson A (1965) The fluorimetric determination of oxalic acid in blood and other biological materials. Biochem J 96:717–721Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • M. Bishop
    • 1
  • H. Freudiger
    • 2
    • 3
  • U. Largiadèr
    • 2
    • 3
  • J. D. Sallis
    • 4
  • R. Felix
    • 2
    • 3
  • H. Fleisch
    • 2
    • 3
  1. 1.Department of UrologyCity HospitalNottinghamEngland
  2. 2.Department of PathophysiologyUniversity of BerneBerneSwitzerland
  3. 3.Department of UrologyInselspitalBerneSwitzerland
  4. 4.Department of BiochemistryUniversity of Tasmania, G. P. O.HobartAustralia

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