Coral Reefs

, Volume 11, Issue 3, pp 143–145 | Cite as

Three methods for isolating viable anthozoan endoderm cells with their intracellular symbiotic dinoflagellates

  • R. D. Gates
  • L. Muscatine


Three maceration methods are described for the isolation of single endoderm cells from marine cnidarians. Two are enzymatic treatments suitable for fleshy anthozoans such as sea anemones and zoanthids. The third employs calcium free sea water and is suitable for stony corals. The viability and morphology of the endoderm cells is described using fluorogenic dyes and scanning and transmission electron microscopy.


Calcium Microscopy Electron Microscopy Transmission Electron Microscopy Dinoflagellate 
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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • R. D. Gates
    • 1
  • L. Muscatine
    • 1
  1. 1.Department of biologyUniversity of CaliforniaLos AngelesUSA

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