Theoretical and Applied Genetics

, Volume 68, Issue 5, pp 467–473 | Cite as

Differential regulation of waxy gene expression in rice endosperm



In order to examine the effects of different alleles on the gene expression at the waxy locus, the Wx gene product which controls the synthesis of amylose was isolated from endosperm starch of rice plants and analysed by electrophoretic techniques. The major protein bound to starch granules was absent in most of waxy strains and increased with the number of Wx alleles in triploid endosperms, suggesting that the major protein is the Wx gene product. In addition to wx alleles which result in the absence or drastic reduction of the Wx gene product and amylose, differentiation of Wx alleles seemed to have occurred among nonwaxy rice strains. At least two Wx alleles with different efficiencies in the production of the major protein as well as amylose were detected. These alleles are discussed in relation to regulation of the gene expression.

Key words

Oryza sativa O. glaberrima Wx gene expression Amylose content Electrophoresis 


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  1. Amano E (1980) Phenotypic expression of Wx gene in cereals. Annu Rep Natl Inst Genet, Misima 31:82–83Google Scholar
  2. Berg BM van den, Wrjsman HJW, Bianch F (1983) Genetics of the peroxidase isoenzyme in Petunia. 6. Differential temporal expression of prx B alleles. Theor Appl Genet 66: 173–178Google Scholar
  3. Bollich CN, Webb BD (1973) Inheritance of amylose of two hybrid populations of rice. Cereal Chem 50:631–636Google Scholar
  4. Echt CS, Schwartz D (1981) Evidence for the inclusion of controlling elements within the structural gene at the waxy locus in maize. Genetics 99:275–284Google Scholar
  5. Endo T (1981) Differential regulation of peroxidase isozymes coded by Px-1 locus in rice. Jpn J Genet 56:175–183Google Scholar
  6. Freeling M, Cheng DSK (1978) Radiation-induced alcohol dehydrogenase mutants in maize following allyl alcohol selection of pollen. Genet Res 31:107–129Google Scholar
  7. Ghosh AK, Govindaswamy S (1972) Inheritance of starch-iodine-blue value and alkali digestion value in rice and their genetic association. Il Riso 21:123–132Google Scholar
  8. Ikeno S (1914) Über die Bestäubung und die Bastardierung von Reis. Z Pflanzenzücht 2:495–503Google Scholar
  9. International Rice Research Institute (1974) Annu Rep 1973. Los Banos, Philippines, pp 3–9Google Scholar
  10. International Rice Research Institute (1976) Annu Rep 1975. Los Banos, Philippines, pp 85–86Google Scholar
  11. Iwata N, Omura T (1971) Linkage analysis by reciprocal translocation method in rice plants (Oryza sativa L.). 1. Linkage groups corresponding to the chromosome 1, 2, 3, and 4. Jpn J Breed 21:19–28Google Scholar
  12. Kudo M (1968) Genetical and breeding studies on physiological and ecological characters in hybrids between ecological groups of rice. Bull Natl Inst Agric Sci, Ser D 19:1–84Google Scholar
  13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685PubMedGoogle Scholar
  14. Li HW, Wang S, Yeh PZ (1965) A preliminary note on the fine structure analysis of glutinous gene in rice. Bot Bull Acad Sin 6:101–105Google Scholar
  15. Li HW, Wu PH, Wu L, Chu MY (1968) Further studies of the interlocus recombination of the glutinous gene in rice. Bot Bull Acad Sin 9:22–26Google Scholar
  16. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–274PubMedGoogle Scholar
  17. McKenzie KS, Rutger JN (1983) Genetic analysis of amylose content, alkali spreading score, and grain dimensions in rice. Crop Sci 23:306–313Google Scholar
  18. Nagao S, Takahashi ME (1963) Trial construction of twelve linkage groups in Japanese rice (Genetical studies on rice plant XXVII). J Fac Agric, Hokkaido Univ 53:72–130Google Scholar
  19. Nelson OE, Chourey PS, Chang MT (1978) Nucleoside diphosphate sugar-starch glucosyl transferase activity of wx starch granules. Plant Physiol 62:383–386Google Scholar
  20. Nelson OE, Rines HW (1962) The enzymatic deficiency in the waxy mutant of maize. Biochem Biophys Res Commun 9:297–300Google Scholar
  21. O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021Google Scholar
  22. Oka HI (1977) Genetic variations of Oryza glaberrima, their survey and evaluation. In: Meeting on African Rice Species, IRAT-ORSTOM, Paris, pp 77–82Google Scholar
  23. Okuno K (1978) Gene dosage effect of waxy alleles on amylose content in endosperm starch of rice. Jpn J Genet 53:219–222Google Scholar
  24. Okuno K, Fuwa H, Yano M (1983) A new mutant gene lowering amylose content in endosperm starch of rice. Jpn J Breed 33:387–394Google Scholar
  25. Sano Y (1978) Gene markers obtained in O. glaberrima. Annu Rep Natl Inst Genet, Misima, 29:97–98Google Scholar
  26. Satoh H, Omura T (1981) New endosperm mutations induced by chemical mutagens in rice, Oryza sativa L. Jpn J Breed 31:316–326Google Scholar
  27. Scandalios JG, Baum JA (1982) Regulatory gene variation in higher plants. Adv Genet 21:347–370Google Scholar
  28. Schwartz D (1966) The genetic control of alcohol dehydrogenase in maize: gene duplication and repression. Proc Natl Acad Sci USA 56:1431–1436Google Scholar
  29. Schwartz D, Endo T (1966) Alcohol dehydrogenase polymorphisms in maize-simple and compound loci. Genetics 53:709–715Google Scholar
  30. Schwartz D, Echt CS (1982) The effect of Ac dosage on the production of multiple forms of Wx protein by the wx m-9 controlling element mutation in maize. Mol Gen Genet 187:410–413Google Scholar
  31. Tsai CY (1974) The function of the waxy locus in starch synthesis in maize endosperm. Biochem Genet 11:83–96Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Y. Sano
    • 1
  1. 1.National Institute of GeneticsMisimaJapan

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