Applied Microbiology and Biotechnology

, Volume 25, Issue 3, pp 300–304

Adaptation of Candida shehatae and Pichia stipitis to wood hydrolysates for increased ethanol production

  • Sarad R. Parekh
  • Shiyuan Yu
  • Morris Wayman
Rapid Communication

Summary

Candida shehatae ATCC 22984 and Pichiastipitis CBS 5776 were tested for ethanol production from xylose, glucose-xylose mixtures, and aspen wood total hydrolysates. Adaptation of these yeasts to wood hydrolysate solutions by recycling resulted in improved substrate utilization and ethanol production. Compared to the non-adapted cultures, recycled C.shehatae and P.stipitis in aspen hydrolysate increased g ethanol/g sugar consumed from 0.39 and 0.41 to 0.45 and 0.47; while ethanol production from a 70:30 glucose-xylose solution (total sugars 140 g/L) was 45 g/L in 24 h and 60 g/L in 72 h with the adapted yeasts compared to 15 g/L and 28 g/L in the same times with the parent strains.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Azhar, A.F., Bery, M.K., Colcord, A.R. and Roberts, R.S. (1982) Development of a yeast strain for the efficient ethanol fermentation of wood acid hydrolysate. Dev Ind Microbiol 23:351–360.Google Scholar
  2. du Preez, J.C. and van der Walt, J.P. (1983) Fermentation of D-xylose to ethanol by a strain of Candida shehatae. Biotechnol Lett 5:357–362.Google Scholar
  3. Fein, J.E., Tallim, R.S. and Lawford, R.G. (1984) Evaluation of D-xylose fermenting yeasts for utilization of a wood-derived hemicellulose hydrolysate. Can J Microbiol 30:682–690.Google Scholar
  4. Gong, C.S. and Tsao, G.T. (1983) Conversion of D-xylose to ethanol by yeasts. In: International Symposium on Ethanol from Biomass, Oct. 13–15, 1982 Winnipeg, Edited by H.E. Duckworth and E.A. Thompson, Royal Society of Canada, Ottawa 525–555.Google Scholar
  5. Maleszka, R., Veliky, A.I. and Schneider, H. (1981) Enhanced rate of ethanol production from D-xylose using recycled or immobilized cells of Pachysolen tannophilus. Biotechnol Lett 3:415–420.Google Scholar
  6. Miller, G.L. (1959) Use of Dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 3:426–428.Google Scholar
  7. Slinginger, P.J., Bothast, R.J., Okos, J.R. and Ladish, M.R. (1985) Comparative evaluation of ethanol production by xylose-fermenting yeasts presented high xylose concentrations. Biotechnol Lett 7:431–436.Google Scholar
  8. Wayman, M. and Parekh, S. (1985) Ethanol and sugar tolerance of Candida shehatae. Biotechnol Lett 7:909–912.Google Scholar
  9. Wayman, M. and Tallevi, A. and Winsborrow, B. (1984) Hydrolysis of biomass by sulphur dioxide. Biomass 6:183–191.Google Scholar
  10. Wayman, M. and Tsuyuki, S. (1985) Fermentation of xylose to ethanol by Candida shehatae. Biotechnol and Bioeng, Symp. No. 15, 167–177.Google Scholar
  11. Yu, S. and Wayman, M. (1986) Fermentation of spent sulphite liquor to butanol and ethanol. J Pulp and Paper Sci 12(3) J 72–77.Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Sarad R. Parekh
    • 1
  • Shiyuan Yu
    • 1
  • Morris Wayman
    • 1
  1. 1.Department of Chemical Engineering and Applied ChemistryUniversity of TorontoTorontoCanada

Personalised recommendations