Applied Microbiology and Biotechnology

, Volume 26, Issue 5, pp 456–461

Ergosterol content of Rhizopus oligosporus NRRL 5905 grown in liquid and solid substrates

  • M. J. R. Nout
  • T. M. G. Bonants-van Laarhoven
  • P. de Jongh
  • P. G. de Koster
Applied Microbiology

Summary

The ergosterol content of Rhizopus oligosporus NRRL 5905 varied between 2–24 μg/ mg biomass dry matter when grown in laboratory media and was found to be influenced by the substrate composition.

When grown on a natural substrate (soya beans) the ergosterol content was considerably higher (estimated at approx. 60–90 μg/mg biomass dry matter).

In laboratory media, the ergosterol content was also influenced by the extent of aeration and the growth phase of the mycelium; within the range of 25° C–35° C, the incubation temperature did not influence the ergosterol content significantly.

In view of these variations, ergosterol should not be used as a chemical index for the quantification of biomass grown in static solid-substrate fermentations with limited mass transfer, e.g. tempe or oncom.

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • M. J. R. Nout
    • 2
  • T. M. G. Bonants-van Laarhoven
    • 2
  • P. de Jongh
    • 2
  • P. G. de Koster
    • 1
  1. 1.DMV CampinaVeghelThe Netherlands
  2. 2.Department of Food ScienceAgricultural UniversityWageningenThe Netherlands

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