Applied Microbiology and Biotechnology

, Volume 26, Issue 5, pp 456–461 | Cite as

Ergosterol content of Rhizopus oligosporus NRRL 5905 grown in liquid and solid substrates

  • M. J. R. Nout
  • T. M. G. Bonants-van Laarhoven
  • P. de Jongh
  • P. G. de Koster
Applied Microbiology
Received:
Revised:

Summary

The ergosterol content of Rhizopus oligosporus NRRL 5905 varied between 2–24 μg/ mg biomass dry matter when grown in laboratory media and was found to be influenced by the substrate composition.

When grown on a natural substrate (soya beans) the ergosterol content was considerably higher (estimated at approx. 60–90 μg/mg biomass dry matter).

In laboratory media, the ergosterol content was also influenced by the extent of aeration and the growth phase of the mycelium; within the range of 25° C–35° C, the incubation temperature did not influence the ergosterol content significantly.

In view of these variations, ergosterol should not be used as a chemical index for the quantification of biomass grown in static solid-substrate fermentations with limited mass transfer, e.g. tempe or oncom.

Keywords

Biomass Fermentation Mass Transfer Aeration Growth Phase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • M. J. R. Nout
    • 2
  • T. M. G. Bonants-van Laarhoven
    • 2
  • P. de Jongh
    • 2
  • P. G. de Koster
    • 1
  1. 1.DMV CampinaVeghelThe Netherlands
  2. 2.Department of Food ScienceAgricultural UniversityWageningenThe Netherlands

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