Applied Microbiology and Biotechnology

, Volume 22, Issue 6, pp 424–427 | Cite as

Use of controlled-release polymer to feed ammonium to Streptomyces clavuligerus cephalosporin fermentations in shake flasks

  • C. Lübbe
  • A. L. Demain
  • K. Bergman
Rapid Communication

Summary

Controlled-release polymers containing NH4Cl were used to feed NH4+to Streptomyces clavuligerus cultures making cephalosporins in shake flasks. Production was improved in comparison to free NH4Cl included in the medium at various concentrations; it approached the performance of l-asparagine, the best single nitrogen source for production.

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References

  1. Aharonowitz Y, Demain AL (1979) Nitrogen nutrition and regulation of cephalosporin production in Streptomyces clavuligerus. Can J Microbiol 25:61–67Google Scholar
  2. Braña AF, Wolfe S, Demain AL (1985) Ammonium repression of cephalosporin production by Streptomyces clavuligerus. Can J Microbiol (in press)Google Scholar
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  7. Weatherburn MW (1967) Phenol-hypochlorite reaction for determination of ammonia. Anal Chem 39:971–974Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • C. Lübbe
    • 1
  • A. L. Demain
    • 1
  • K. Bergman
    • 1
  1. 1.Fermentation Microbiology Laboratory, Department of Applied Biological SciencesMassachusetts Institute of TechnologyCambridgeUSA

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