The effect of antimetabolites on the microbial biosynthesis of vitamin B12 and related compounds
Applied Microbiology
Received:
Revised:
- 32 Downloads
- 1 Citations
Summary
Two compounds which are both antimetabolites and precursors of vitamin B12, o-phenylendiamine, and 5,6-dimethylbenzimidazole, stimulated the production of vitamin B12 by Propionibacterium freudenreichii at concentrations which were subinhibitory for growth. The stimulatory effect of the compounds depended not only on their concentration, but also on the time of addition. During cultivation, two chromatographically distinguishable fractions with vitamin B12 activity were formed. At concentrations which stimulated production of vitamin B12, only the biosynthesis of true vitamin B12 (cyanocobalamin) took place, while the biosynthesis of the analogue with a higher molecular weight was inhibited.
Keywords
Molecular Weight High Molecular Weight Related Compound Cyanocobalamin Distinguishable Fraction
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Preview
Unable to display preview. Download preview PDF.
References
- Fantes KH, O'Callaghan CH (1954) The biosynthesis of a new vitamin B12 analogue. Biochem J 58: Proc XXIGoogle Scholar
- Fantes KH, O'Callaghan CH (1955) A new vitamin B12 analogue. Biochem J 59:79–82Google Scholar
- Fantes KH, O'Callaghan CH, Goodinson DW (1956) A note on the comparison of the microbial potencies of two different growth factors with special reference to the assay of desdimethylcyanocobalamin. Biochim Biophys Acta 20:379–398Google Scholar
- Ford JE, Haldsworth ES, Kon SK (1954) Biosynthesis of vitamin B12 like compounds. Biochem J 58: Proc XXIVGoogle Scholar
- Goodwin TW (1963) The biosynthesis of vitamins and related compounds, 1st edn. Academic Press, New YorkGoogle Scholar
- Hallinger LN, Silber R, Neumann G (1954) Inhibitory effect of 5,6-dimethylbenzimidazole and of 1,2-dimethyl-4,5-diaminobenzene on growth of Mycobacterium tuberculosis. Proc Soc Exp Biol Med 85:624–626Google Scholar
- Hendlin D, Soars MH (1951) The effect of 5,6-dimethyl-benzimidazole and related compounds on the growth of Lactobacillus lactis Dorner. J Bacteriol 62:633–638Google Scholar
- Králová B, Rauch P, Černá J (1983) Use of vitamin B12 radioassay in the analysis of biological materials, mainly of foods. Nahrung 26:803–810Google Scholar
- Sahashi Y, Mikata M, Sakao H (1950) Biochemical studies on vitamin B12. Bull Chem Soc Japan 23:247–249Google Scholar
- Šícho V, Králová B (1966a) Effect of antivitamins on the microbial biosynthesis of vitamins. Sci Papers Inst Chem Technol Prague E9:75–80Google Scholar
- Šícho V, Králová B (1966b) Stimulation of nicotinic acid biosynthesis in the microorganism Saccharomyces cerevisiae by means of pyridine-3-sulphonic acid. Sci Papers Inst Chem Technol Prague E11:9–13Google Scholar
- Šícho V, Králová B (1968) Effect of antivitamins on the microbiological biosynthesis of vitamins, III. Relation between amount of thiamine, thiamine monophosphate and thiamine diphosphate in S. cerevisiae under normal conditions and with oxythiamine in culture medium. Biochim Biophys Acta 165:459–462Google Scholar
- Šícho V, Králová B (1969) Effect of antivitamins on the microbial biosynthesis of vitamins. IV. Effect of oxythiamine on the thiamine phosphorylation by the thiamine pyrophosphokinase of yeast. Biochim Biophys Acta 184:207–209Google Scholar
- Woolley DW (1950) Inhibition of synthesis of vitamin B12 and of riboflavin by 1,2-dichloro-4,5-diaminobenzene in bacterial cultures. Proc Soc Exp Biol Med 75:745–746Google Scholar
- Woolley DW (1953) Evidence for the synthesis of vitamin B12 by spontaneous tumors. Proc Nat Acad Sci USA 39:6–18Google Scholar
Copyright information
© Springer-Verlag 1985