Applied Microbiology and Biotechnology

, Volume 20, Issue 2, pp 111–117 | Cite as

Ethanol production during D-xylose, L-arabinose, and D-ribose fermentation by Bacteroides polypragmatus

  • Girishchandra B. Patel


The specific growth rate (μ) during cultivation of Bacteroides polypragmatus in 2.51 batch cultures in 4–5% (w/v) l-arabinose medium was 0.23 h-1 while that in either d-xylose or d-ribose medium was lower (μ=0.19 h-1). Whereas growth on arabinose or xylose occurred after about 6–8 h lag period, growth on ribose commenced after a 30 h lag phase. The maximum substrate utilization rate for arabinose, ribose and xylose in media with an initial substrate concentration of 4–5% (w/v) was 0.77, 0.76, and 0.60 g/l/h respectively. In medium containing a mixture of glucose, arabinose, and xylose, the utilization of all three substrates occurred concurrently. The maximum amount of ethanol produced after 72 h growth in 4–5% (w/v) of arabinose, xylose, and ribose was 9.4, 6.5, and 5.3 g/l, respectively. The matabolic end products (mol/mol substrate) of growth in 4.4% (w/v) xylose medium were 0.73 ethanol, 0.49 acetate, 1.39 CO2, 1.05 H2, and 0.09 butyrate.


Fermentation Xylose Butyrate Ethanol Production Specific Growth Rate 


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  1. Ackman RG (1972) Porous polymer bead packings and formic acid vapour in the GLC of volatile free fatty acids. J Chromatogr Sci 10:560–565Google Scholar
  2. Baillargeon MW, Jansen NB, Gong CS, Tsao GT (1983) Effect of oxygen uptake rate on ethanol production by a xylose-fermenting yeast mutant, Candida sp. XF217. Biotechnol Lett 5:339–344Google Scholar
  3. Bull AT, Holt G, Lilly MD (1982) Biotechnology. International trends and perspectives. Organisation for Economic Cooperation and Development, Paris, pp 11–29Google Scholar
  4. Detroy RW, Cunningham RL, Bothast RJ, Bagby MO, Herman A (1982) Bioconversion of wheat straw cellulose/hemicellulose to ethanol by Saccharomyces ovarum and Pachysolen tannophilus. Biotechnol Bioeng 24:1105–1113Google Scholar
  5. Dunning JW, Lathrop EC (1945) The saccharification of agricultural residues: a continuous process. Ind Eng Chem 37:24–29Google Scholar
  6. Du Preez JC, Van der Walt JP (1983) Fermentation of d-xylose to ethanol by a strain of Candida shehatae. Biotechnol Lett 5:357–362Google Scholar
  7. Flickinger MC (1980) Current biological research in conversion of cellulosic carbohydrates into liquid fuels: how far have we come. Biotechnol Bioeng (Suppl 1) 22:27–48Google Scholar
  8. Gong CS, Ladisch MR, Tsao GT (1981a) Production of ethanol from wood hemicellulose hydrolysates by a xylose-fermenting yeast mutant, Candida sp. XF217. Biotechnol Lett 3:657–662Google Scholar
  9. Gong CS, Maun CM, Tsao GT (1981b) Direct fermentation of cellulose to ethanol by a cellulolytic filamentous fungus, Monilia sp. Biotechnol Lett 3:77–82Google Scholar
  10. Gong CS, McCracken LD, Tsao GT (1981c) Direct fermentation of d-xylose to ethanol by a xylose-fermenting yeast mutant, Candida sp. XF217. Biotechnol Lett 3:245–250Google Scholar
  11. Gonzalez-Valdes A, Moo-Young M (1981) Production of yeast SCP from corn stover hydrolysates. Biotechnol Lett 3:143–148Google Scholar
  12. Holdeman LV, Moore WEC (1975) Anaerobe laboratory manual, 3rd edn, Virginia Polytechnic Institute and State University, Blackburg, Virginia, pp 125–126Google Scholar
  13. Jeffries TW (1981) Conversion of xylose to ethanol under aerobic conditions by Candida tropicalis. Biotechnol Lett 3:213–218Google Scholar
  14. Khan AW, Murray WD (1982) Single step conversion of cellulose to ethanol by a mesophilic coculture. Biotechnol Lett 4:177–180Google Scholar
  15. Kosaric N, Wieczorek A, Cosentino GP, Magee RJ, Prenosil JE (1983) Ethanol fermentation. In: Dellweg H (ed) Biotechnology, vol 3. Verlag Chemie, Weinheim, pp 257–385Google Scholar
  16. Margaritis A, Bajpai P (1982) Direct fermentation of d-xylose to ethanol by Kluyveromyces marxianus strains. Appl Environ Microbiol 44:1039–1041Google Scholar
  17. Murray WD, Khan AW (1983a) Ethanol production by a newly isolated anaerobe, Clostridium saccharolyticum: effects of culture medium and growth conditions. Can J Microbiol 29:342–347Google Scholar
  18. Murray WD, Khan AW (1983b) Growth requirements of Clostridium saccharolyticum, an ethanologenic anaerobe. Can J Microbiol 29:348–353Google Scholar
  19. Patel GB (1983) Fermentation of lactose by Bacteroides polypragmatus. Can J Microbiol 29:120–128Google Scholar
  20. Patel GB, Breuil C (1981) Isolation and characterization of Bacteroides polypragmatus sp. nov., an isolate that produces carbon dioxide, hydrogen and acetic acid during growth on various organic substrates. In: Moo-Young M, Robinson CW (eds) Advances in biotechnology, vol 2. Pergamon Press, Toronto, pp 291–296Google Scholar
  21. Patel GB, Roth LA (1978) Acetic acid and hydrogen metabolism during coculture of an acetic acid producing bacterium with methanogenic bacteria. Can J Microbiol 24:1007–1010Google Scholar
  22. Rosenberg SL (1980) Fermentation of pentose sugars to ethanol and other neutral products by microorganisms. Enzyme Microbiol Technol 2:185–193Google Scholar
  23. Rosenberg SL, Batter TR, Blanch HW, Wilke CR (1981) Hemicellulose utilization for ethanol production. The Am Inst Chemical Engineers Symp 77:107–114Google Scholar
  24. Schneider H, Wang PY, Chan YK, Maleszka R (1981) Conversion of d-xylose into ethanol by the yeast Pachysolen tannophilus. Biotechnol Lett 3:89–92Google Scholar
  25. Sciamanna AF, Freitas RP, Wilke CR (1977) Composition and utilization of cellulose for chemicals from agricultural residues. Lawrence Berkley Laboratory Report No. 5966, Berkley, California, USAGoogle Scholar
  26. Slininger PJ, Bothast RJ, Van Cauwenberge JE, Kurtzman CP (1982) Conversion of d-xylose to ethanol by the yeast Pachysolen tannophilus. Biotechnol Bioeng 24:371–384Google Scholar
  27. Standing CN, Fredrickson AG, Tsuchiya HM (1972) Batch and continuous-culture transients for two substrate systems. Appl Microbiol 23:354–359Google Scholar
  28. Ueng PP, Gong CS (1982) Ethanol production from pentoses and sugar-cane bagasse hemicellulose hydrolysate by Mucor and Fusarium species. Enzyme Microbiol Technol 4:169–171Google Scholar
  29. van Huyssteen JJ (1967) Gas chromatographic separation of digester gases using porous polymers. Water Res 1:237–242Google Scholar
  30. Wang DIC, Biocic I, Fang HY, Wang SD (1979) Direct microbiological conversion of cellulose biomass to ethanol. Proc 3rd Annual Biomass Energy Systems Conf. Golden, Colorado. National Technical Information Service, US Dept of Commerce, pp 61–67Google Scholar
  31. Wolin EA, Wolin MJ, Wolfe RS (1963) Formation of methane by bacterial extracts. J Biol Chem 238:2882–2886Google Scholar
  32. Yang HJ, Hakomori SI (1971) A sphingolipid having a novel type of ceramide and lacto-N-fucopentaose III. J Biol Chem 246:1192–1200Google Scholar
  33. Yu EKC, Saddler JN (1982) Power solvent production by Klebsiella pneumoniae grown on sugars present in wood hemicellulose. Biotechnol Lett 4:121–126Google Scholar
  34. Zehnder AJB, Wuhrmann K (1976) Titanium (III) citrate as a non-toxic oxidation-reduction buffering system for the culture of obligate anaerobes. Science 194:1165–1166Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Girishchandra B. Patel
    • 1
  1. 1.Division of Biological SciencesNational Research Council of CanadaOttawaCanada

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