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Diabetologia

, Volume 26, Issue 1, pp 30–33 | Cite as

Cell-mediated cytotoxic islet cell surface antibodies to human pancreatic beta cells

  • T. Maruyama
  • I. Takei
  • I. Matsuba
  • A. Tsuruoka
  • M. Taniyama
  • Y. Ikeda
  • K. Kataoka
  • M. Abe
  • S. Matsuki
Originals

Summary

Sera containing islet cell surface antibodies show a complement-dependent cytotoxic reaction against islet cells, but it has not yet been clarified whether islet cell surface antibodies exhibit cell-mediated cytotoxicity to these cells. By 51Cr release assay we investigated whether islet cell surface antibodies showed a cytotoxic reaction to human pancreatic B cells (JHPI-1 clone) in the presence of normal human lymphocytes. The sera from 14 islet cell surface antibody-positive, 16 islet cell surface antibody-negative Type 1 (insulin-dependent) diabetic patients and 18 islet cell surface antibody-negative healthy subjects were studied. Four sera containing islet cell surface antibodies showed specific cytotoxicity above the mean +3SD value of healthy subjects, and the mean specific cytotoxicity of islet cell surface antibody-positive sera differed significantly from that of both islet cell surface antibody-negative groups. These results suggest that this cell-mediated cytotoxic mechanism may play an important role in the pathogenesis of Type 1 diabetes.

Key words

Type 1 diabetes islet cell surface antibodies cytotoxic antibodies antibody-dependent cell-mediated cytotoxcity human pancreatic B cells islet cells 

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • T. Maruyama
    • 1
  • I. Takei
    • 1
  • I. Matsuba
    • 2
  • A. Tsuruoka
    • 2
  • M. Taniyama
    • 1
  • Y. Ikeda
    • 2
  • K. Kataoka
    • 1
  • M. Abe
    • 2
  • S. Matsuki
    • 1
  1. 1.Department of Internal MedicineSchool of Medicine, Keio UniversityTokyoJapan
  2. 2.Third Department of Internal MedicineJikei University School of MedicineTokyoJapan

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