, Volume 26, Issue 1, pp 24–29 | Cite as

Clinical time-course and characteristics of islet cell cytoplasmatic antibodies in childhood diabetes

  • G. J. Bruining
  • J. Molenaar
  • C. W. Tuk
  • J. Lindeman
  • H. A. Bruining
  • B. Marner


Circulating islet cell antibodies (ICA) were present in high frequency (80%) early after diagnosis and decreased in the time course of childhood diabetes mellitus. The complement fixing ability of islet cell antibodies (CF-ICA) in the course of the disease appeared to depend on the titre of ICA: the coefficient of correlation between ICA and CF-ICA titres was 0.79 and all ICA's with a titre over 16 were complement-fixing. Incubating fresh frozen human pancreatic sections thrice rather than once with the children's sera, increased the detectability of complement fixation by a factor 1.4 in all ICA-positive sera. Thus tested, the detection of complement fixation per se did not appear to have a separate pathogenic significance, as the fraction of complement fixing ICA's was almost constant throughout the clinical course. The presence of ICA-IgG subclasses also was dependent on the ICA titre: above a titre of 16 mostly all four subclasses could be detected. Incubating the pancreatic tissue thrice rather than once with ICA-positive sera resulted in enhanced detectability of ICA-IgG1. Early in the course of childhood diabetes, including two prediabetic children, most of the IgG subclasses could be detected in ICA, but after a duration of one year IgG1 alone was mainly seen. In two other children, having a family history of insulin-dependency, restriction to the IgG2 subclass was found.

Key words

Juvenile diabetes mellitus autoantibodies fluorescent antibody technique prediabetic state IgG 


  1. 1.
    Gorsuch AN, Spencer KM, Lister J, Wolf E, Bottazzo GF, Cudworth AG (1982) Can future Type 1 diabetes be predicted? A study of families of affected children. Diabetes 31: 862–867Google Scholar
  2. 2.
    Van de Winkel M, Smets G, Gepts W, Pipeleers D (1982) Islet cell surface antibodies from insulin-dependent diabetics bind specifically to pancreatic B cells. J Clin Invest 70: 41–49Google Scholar
  3. 3.
    Lernmark A, Hägglöf B, Freedman ZR, Irvine WJ, Ludvigsson J, Holmgren G (1981) A prospective analysis of antibodies reactive with pancreatic islet cells in insulin-dependent diabetic children. Diabetologia 20: 471–474Google Scholar
  4. 4.
    Marner B, Lernmark A, Nerup J, Molenaar JL, Tuk CW, Bruining GJ (1983) Analysis of islet cell antibodies on sections of human pancreas. Diabetologia 25: 93–96Google Scholar
  5. 5.
    Estes D, Atra E, Peltier A (1973) An immunofluorescent method for the detection of antigamma-globulin antibodies. Arthritis Rheum 16: 1–9Google Scholar
  6. 6.
    Van der Giessen M, Freyee W, Rossouw E, van Loghem E (1973) Qualitative and quantitative studies on IgG2 globulins in individual human sera with an antiserum capable of differentiating between Gm (n+) and Gm (n-) proteins. Clin Exp Immunol 14: 127–132Google Scholar
  7. 7.
    Von dem Borne AEGK, Helmerhorst FM, van Leeuwen EF, Peels HG, van Riesz E, Engelfriet CP (1980) Autoimmune thrombocytopenia: detection of platelet antibodies with the suspension immunofluorescence test. Br J Haematol 45: 319–327Google Scholar
  8. 8.
    Horai S, Class FHJ, van Rood JJ (1981) Detection of platelet antibodies by enzyme linked immunosorbent assay (Elisa). Comparative studies with the indirect immunofluorescence assay. Immunol Lett 3: 155–158Google Scholar
  9. 9.
    Dean BM, Bottazzo GF, Cudworth AG (1983) IgG-subclass distribution in organ specific autoantibodies. The relationship to complement-fixing ability. Clin Exp Immunol 52: 61–66Google Scholar
  10. 10.
    Huen AHJ, Haneda M, Freedman Z, Lernmark A, Rubenstein AH (1983) Quantitative determination of islet cell surface antibodies using 125I-protein A. Diabetes 32: 460–465Google Scholar
  11. 11.
    Baekesshov S, Nielsen JH, Marner B, Bilde T, Ludvigsson J, Lernmark A (1982) Autoantibodies in newly diagnosed diabetic children immunoprecipitate human pancreatic islet cell proteins. Nature 298: 167–169Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • G. J. Bruining
    • 1
  • J. Molenaar
    • 2
  • C. W. Tuk
    • 2
  • J. Lindeman
    • 2
  • H. A. Bruining
    • 3
  • B. Marner
    • 4
  1. 1.Department of PediatricsErasmus University and University Hospital Rotterdam/Sophia Children's HospitalRotterdam
  2. 2.Department of Clinical ImmunologyStichting Samenwerking Delftse ZiekenhuizenDelft
  3. 3.Department of SurgeryErasmus University and University HospitalRotterdamThe Netherlands
  4. 4.Hagedorn Research LaboratoryGentofteDenmark

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