Characterization of Gluconobacter oxydans immobilized in calcium alginate

  • Patrick Adlercreutz
  • Olle Holst
  • Bo Mattiasson
Biotechnology
Received:

Summary

Gluconobacter oxydans cells were immobilized in calcium alginate and the preparation was used for the oxidation of glycerol to dihydroxyacetone. The characterization was done according to the guidelines given by the Working Party on Immobilized Biocatalysts of the European Federation of Biotechnology. The pH optimum of the preparation was found to be 5.0 and the temperature optimum was 40°C. However, the operational stability was better at 30°C. The glycerol concentration required to obtain half the maximal reaction rate was about 5 mM for both immobilized and free cells. At low concentrations of glycerol and high concentrations of dihydroxyacetone a slight inhibition was noted. No loss of activity of the immobilized preparation was observed after storage for 68 days at +4°C. Investigation of the operational stability revealed a half-life of 5 days. Studies of the influence of particle size and cell densities as well as that of oxygen concentration revealed that the oxygen supply was the rate limiting step.

Keywords

Particle Size Glycerol Cell Density Alginate Oxygen Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Patrick Adlercreutz
    • 1
  • Olle Holst
    • 2
  • Bo Mattiasson
    • 1
  1. 1.Department of Biotechnology; Chemical CenterUniversity of LundLundSweden
  2. 2.Department of Applied Microbiology; Chemical CenterUniversity of LundLundSweden

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