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Applied Microbiology and Biotechnology

, Volume 25, Issue 6, pp 513–520 | Cite as

Culture conditions for growth and solvent biosynthesis by a modified Clostridium acetobutylicum

  • L. Yerushalmi
  • B. Volesky
Biotechnology

Summary

A modified strain of Clostridium acetobutylicum and the fermentation medium conditions for good growth of the culture and normal production of solvents are described. The pretreatment of the culture with butyric-acid-enriched medium increased the final solvent yield on sugar and lowered the residual butyric acid accumulation. In a complex medium, relatively high concentrations of yeast extract (7.5 g/l) and ammonium sulphate (3 g/l to 6 g/l) were required for normal solvent synthesis. The nitrogen requirements for cellular growth and solvent production were distinctively different. Production of solvents and growth of the culture were dependent on the concentration of para-aminobenzoic acid and relatively independent of the variations of the initial pH of the medium in the range of 4.6 to 6.3. Solvent production was obtained with initial glucose concentrations of 20.5 g/l to 70 g/l, resulting in a maximum solvent concentration of 22 g/l and a maximum yield on glucose of 32.7%.

Keywords

Fermentation Clostridium Butyric Acid Fermentation Medium Cellular Growth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • L. Yerushalmi
    • 1
  • B. Volesky
    • 1
  1. 1.Biochemical Engineering Research Unit, Department of Chemical EngineeringMcGill UniversityMontrealCanada

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