Applied Microbiology and Biotechnology

, Volume 27, Issue 2, pp 214–217 | Cite as

Hudson River sediments as a source of actinomycetes exhibiting antifungal activity

  • Michael A. Pisano
  • Michael J. Sommer
  • Brian P. Brett
Environmental Microbiology

Summary

Actinomycetes were isolated from sediments obtained from the Hudson River. Pretreatments utilized to improve the recovery of these microorganisms included heat and exposure to phenol or benzalkonium chloride. In addition, plating of sediment samples on selective agar substrates was also employed. These pretreatments eliminated or severely limited the growth of contaminating microorganisms thereby facilitating the isolation of actinomycetes. Of 165 isolates obtained, 22 exhibited significant antimycotic activity following growth in submerged culture. Among the test fungi examined, Candida krusei and Trichoderma viride proved to be the most susceptible to the active substances present in the fermentation broths. All but three of the latter contained polyenes. With one exception, the bioactive actinomycetes were identified as streptomycetes.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Becker B, Lechevalier MP, Gordon RE, Lechevalier HA (1964) Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole cell hydrolysates. Appl Microbiol 12:421–423Google Scholar
  2. Bormann C, Huhn W, Zahner H (1985) Metabolic products of microorganisms. 228 New nikkomycins produced by mutants of Streptomyces tendae. J Antibiotics 38:9–16Google Scholar
  3. Cross T (1982) Actinomycetes: a continuing source of new metabolites. Develop Indust Microbiol 23:1–8Google Scholar
  4. Goodfellow M, Haynes JA (1984) Actinomycetes in marine sediments. In: Ortiz-Ortiz L, Bojalil LF, Yakoleff V (eds) Biological, biochemical, and biomedical aspects of actinomycetes. Academic Press, New York, London, pp 453–472Google Scholar
  5. Hamamoto T, Gunji S, Tsuji H, Beppu T (1983) Leptomycins A and B, new antifungal antibiotics. I. Taxonomy of the producing strain and their fermentation, purification and characterization. J Antibiotics 36:639–645Google Scholar
  6. Hsu SC, Lockwood JL (1975) Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 29:422–426Google Scholar
  7. Ishimaru K, Ishida J, Noborio N, Nakamura S (1983) Histidinomycin, a new antifungal antibotic. J Antibiotics 36:1644–1650Google Scholar
  8. Lawrence CH (1956) A method for isolating actinomycetes from scabby potato tissue and soil with minimal contamination. Can J Botany 34:44–47Google Scholar
  9. Medoff G, Brajtburg J, Kobayashi GS (1983) Antifungal agents useful in therapy of systemic fungal infections. Ann Rev Pharmacol Toxicol 23:303–330Google Scholar
  10. Nonomura H, Ohara Y (1969) Distribution of actinomycetes in soil. VI. A culture method effective for both preferential isolation and enumeration of Microbispora and Streptosporangium strains in soil (Part I). J Ferment Technol 47:463–469Google Scholar
  11. Okami Y (1986) Marine microorganisms as a source of bioactive agents. Microbial Ecol 12:65–78Google Scholar
  12. Omura S, Tanaka Y, Nakagawa A, Iwai Y, Inoue M, Tanaka H (1982) Irumamycin, a new antibiotic active against phytopathogenic fungi. J Antibiotics 35:256–257Google Scholar
  13. Pisano MA, Sommer MJ, Lopez MM (1986) Application of pretreatments for the isolation of bioactive actinomycetes from marine sediments. Appl Microbiol Biotechnol 25:285–288Google Scholar
  14. Rowbotham TJ, Cross T (1977) Ecology of Rhodococcus coprophilus and associated actinomycetes in fresh water and agricultural habitats. J Gen Microbiol 100:231–240Google Scholar
  15. Sasaki T, Furihata K, Shimazu A, Seto H, Iwata M, Watanabe T, Otake N (1986) A novel macrolide antibiotic, notonesomycin A. J Antibiotics 39:502–509Google Scholar
  16. Soliveri J, Arias M-E, Laborda F (1987) PA-5 and PA-7, pentaene and heptaene macrolide antibiotics produced by a new isolate of Streptoverticillium from Spanish soil. Appl Microbiol Biotechnol 25:366–371Google Scholar
  17. Takatsu T, Nakayama H, Shimazu A, Furihata K, Ikeda K, Furihata K, Seto H, Otake N (1985) Rustimicin, a new macrolide antibiotic active against wheat germ stem rust fungus. J Antibiotics 38:1806–1809Google Scholar
  18. Tunac JB, Graham BD, Dobson WE, Lenzini MD (1985) Fermentation by a new daunomycin-producing organism, Streptomyces insignis ATCC 31913. Appl Environ Microbiol 49:265–268Google Scholar
  19. Uramoto M, Shen Y-C, Takizawa N, Kusakabe H, Isono K (1985) A new antifungal antibiotic, phosphazomycin A. J Antibiotics 38:665–668Google Scholar
  20. Veiga M, Fabregas J (1983) Tetrafungin, a new polyene macrolide antibiotic. I. Fermentation, isolation, characterization, and biological properties. J Antibiotics 36:770–775Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Michael A. Pisano
    • 1
  • Michael J. Sommer
    • 1
  • Brian P. Brett
    • 1
  1. 1.Department of Biological SciencesSt. John's UniversityJamaicaUSA

Personalised recommendations