Applied Microbiology and Biotechnology

, Volume 27, Issue 4, pp 333–336 | Cite as

The role of xylonolactone in xylonic acid production by Pseudomonas fragi

  • Johanna Buchert
  • Liisa Viikari


Xylonic acid was produced by Pseudomonas fragi ATCC 4973 on 15% xylose medium with a yield of 96% of the theoretical. In the middle of the fermentation, growth was inhibited due to formation of inhibitory xylono-γ-lactone. The spontaneous, non-enzymatic hydrolysis of lactone accounted for only 19% of the hydrolysis demand, thus explaining the accumulation of xylonolactone in the broth. It appeared that high amounts of xylono-γ-lactone induced xylonolactonase enzyme, which brought about the total hydrolysis of xylonolactone.


Enzyme Hydrolysis Fermentation Xylose Pseudomonas 
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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Johanna Buchert
    • 1
  • Liisa Viikari
    • 1
  1. 1.Biotechnical LaboratoryVTTEspooFinland

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