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Applied Microbiology and Biotechnology

, Volume 27, Issue 4, pp 333–336 | Cite as

The role of xylonolactone in xylonic acid production by Pseudomonas fragi

  • Johanna Buchert
  • Liisa Viikari
Biotechnology

Summary

Xylonic acid was produced by Pseudomonas fragi ATCC 4973 on 15% xylose medium with a yield of 96% of the theoretical. In the middle of the fermentation, growth was inhibited due to formation of inhibitory xylono-γ-lactone. The spontaneous, non-enzymatic hydrolysis of lactone accounted for only 19% of the hydrolysis demand, thus explaining the accumulation of xylonolactone in the broth. It appeared that high amounts of xylono-γ-lactone induced xylonolactonase enzyme, which brought about the total hydrolysis of xylonolactone.

Keywords

Enzyme Hydrolysis Fermentation Xylose Pseudomonas 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Johanna Buchert
    • 1
  • Liisa Viikari
    • 1
  1. 1.Biotechnical LaboratoryVTTEspooFinland

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