A heteroplasmic state induced by protoplast fusion is a necessary condition for detecting rearrangements in Nicotiana mitochondrial DNA
Mitochondrial DNA (mtDNA) restriction patterns were studied in mutant, cybrid and somatic hybrid plants regenerated from Nicotiana protoplasts.
It has been shown that neither components of the culture media used for protoplast culture and plant regeneration, nor the antibiotics streptomycin and lincomycin used for the mutant selection induced alterations in the mtDNA. No rearrangements were detected in the mtDNA of plants derived from homoplasmic fusions where the mtDNA of the parents was identical as judged by mtDNA restriction patterns.
There were rearrangements, however, in the mtDNA of each of the cybrid plants derived from heteroplasmic fusions. Restriction patterns generated by BamHI and SalI restriction endonucleases were different from those of both parents, and were composed of parental and non-parental fragments.
Key wordsNicotiana Mitochondrial DNA-cybrids Somatic hybrids
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- Arrand JR, Myers PA (1978) A new restriction endonuclease from Streptomyces albus G. J Mol Biol 118:127–135Google Scholar
- Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature (London) 281:401–403Google Scholar
- Dale RMK (1981) Sequence homology among different size classes of plant mtDNAs. Proc Natl Acad Sci USA 78: 4453–4457Google Scholar
- Galun E, Arzee-Gonen P, Fluhr R, Edelman M, Aviv D (1982) Cytoplasmic hybridization in Nicotiana: mitochondrial DNA analysis in progenies resulting from fusion between protoplasts having different organelle constitution. Mol Gen Genet 186:50–56Google Scholar
- Gengenbach BG, Conelly JA, Pring DR, Conde MR (1981) Mitochondrial DNA variation in maize plants regenerated during tissue culture selection. Theor Appl Genet 59: 161–167Google Scholar
- Heizmann P, Hussein Y, Nicolas P, Nigon V (1982) Modifications of chloroplast DNA during streptomycin induced mutagenesis in Euglena gracilis. Curr Genet 5:9–15Google Scholar
- Kemble RJ, Flavell RB, Brettel RIS (1982) Mitochondrial DNA analysis of fertile and sterile maize plants derived from tissue cultures with Texas male sterile cytoplasm. Theor Appl Genet 62:213–219Google Scholar
- Linsmaier M, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100–127Google Scholar
- Maliga P, Sz-Breznovits A, Márton L (1973) Streptomycin-resistant plant from callus culture of haploid tobacco. Nature (London), New Biol 244:29–30Google Scholar
- Maliga P, Sz-Breznovits A, Joó F, Márton L (1975) Non-Mendelian streptomycin resistant tobacco mutant with altered chloroplasts and mitochondria. Nature (London) 255:401–402Google Scholar
- Maliga P, Lörz H, Lázár G, Nagy F (1982) Cytoplast-protoplast fusion for interspecific chloroplast transfer in Nicotiana. Mol Gen Genet 185:211–215Google Scholar
- Marton L, Sidorov V, Biasini G, Maliga P (1982) Complementation in somatic hybrids indicates four types of nitrate redictase deficient lines in Nicotiana plumbaginifolia. Mol Gen Genet 187:1–3Google Scholar
- Medgyesy P, Menczel L, Maliga P (1980) The use of cytoplasmic streptomycin resistance: chloroplast transfer from Nicotiana tabacum into Nicotiana sylvestris and isolation of their somatic hybrids. Mol Gen Genet 179:693–698Google Scholar
- Menczel L, Galiba G, Nagy F, Maliga P (1982) Effect of radiation dosage on the efficiency of chloroplast transfer by protoplast fusions in Nicotiana. Genetics 100:487–495Google Scholar
- Nagy F, Török I, Maliga P (1981) Extensive rearrangements in the mitochondrial DNA in somatic hybrids of Nicotiana tabacum and Nicotiana knightiana. Mol Gen Genet 183: 437–439Google Scholar
- Sager R (1972) Cytoplasmic genes and organelles. Academic Press, London New YorkGoogle Scholar
- Sears BB (1980) Elimination of plastids during spermato-genesis and fertilization in the plant kingdom. Plasmid 4:233–255Google Scholar
- Sidorov VA, Menczel L, Nagy F, Maliga P (1981) Chloroplast transfer in Nicotiana based on metabolic complementation between irradiated and iodoacetate treated protoplasts. Planta 152:341–345Google Scholar
- Smith HH (1975) Nicotiana. In: King RC (ed) Handbook of genetics, vol 2/12. Plenum Press, New York, p 281Google Scholar
- Sparks RB, Dale RMK (1980) Characterization on H3-labelled supercoiled mitochondrial DNA from tobacco suspension culture cells. Mol Gen Genet 180:351–355Google Scholar
- Wettstein DV, Eriksson G (1965) The genetics of chloroplasts. Genetics Today 3:591–610Google Scholar
- Wong-Staal F, Wildman SG (1973) Identification of a mutation in chloroplast DNA correlated with formation of defective chloroplasts in a variegated mutant of Nicotiana tabacum. Planta 113:313–326Google Scholar