Theoretical and Applied Genetics

, Volume 69, Issue 5–6, pp 535–541

Comparison of the mitochondrial genome of Nicotiana tabacum with its progenitor species

  • M. M. Bland
  • D. F. Matzinger
  • C. S. LevingsIII
Article

Summary

Mitochondrial DNAs from Nicotiana tabacum, an amphiploid, and its putative progenitor species, N. sylvestris and N. tomentosiformis were compared in structure and organization. By using DNA transfer techniques and cloned fragments of known genes from maize and N. sylvestris as labeled probes, the positions of homologous sequences in restriction digests of the Nicotiana species were analyzed. Results indicate that the mitochondrial DNA of N. tabacum was inherited from N. sylvestris. Conservation in organization and sequence homology between mtDNAs of N. tabacum and the maternal progenitor, N. sylvestris, provide evidence that the mitochondrial genome in these species is evolutionarily stable. Approximately one-third of the probed restriction fragments of N. tomentosiformis mtDNA showed conservation of position with the other two species. Pattern variations indicate that extensive rearrangement of mtDNA has occurred in the evolution of these Nicotiana species.

Key words

Nicotiana Progenitor species mtDNA Restriction endonuclease Cloned fragments 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281:401–403Google Scholar
  2. Burk LG, Durbin RD (1978) The reaction of Nicotiana species to tentoxin. J Hered 69:117–120Google Scholar
  3. Chao S, Sederoff RR, Levings CS III (1983) Partial sequence analysis of the 5S to 18S rDNA gene region of the maize mitochondrial genome. Plant Physiol 71:190–193Google Scholar
  4. Dale RMK, Duesing JH, Keene D (1981) Supercoiled mitochondrial DNAs from plant tissue culture cells. Nucleic Acids Res 9:4583–4593Google Scholar
  5. Dale RMK, Wu M, Kiernan MCC (1983) Analysis of four tobacco mitochondrial DNA size classes. Nucleic Acids Res 11:1673–1685Google Scholar
  6. Dewey RE, Schuster AM, Levings CS III, Timothy DH (1984) Nucleotide sequence of F0-ATPase proteolipid (subunit 9) gene in maize mitochondria. Proc Natl Acad Sci USA (in press)Google Scholar
  7. Fox TD, Leaver CJ (1981) The Zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons. Cell 26:315–323Google Scholar
  8. Galun E, Arzee-Gonen P, Fluhr R, Edelman M, Aviv D (1982) Cytoplasmic hybridization in Nicotiana: mitochondrial DNA analysis in progenies resulting from fusion between protoplasts having different organelle constitutions. Mol Gen Genet 186:50–56Google Scholar
  9. Gerstel DU (1960) Segregation in new allopolyploids of Nicotiana. 1. Comparison of 6X (N. tabacumxtomentosi x formis) and 6X (N. tabacum x otophora). Genetics 45: 1723–1734Google Scholar
  10. Gerstel DU (1976) Tobacco. Nicotiana tabacum (Solanaceae). In: Simmonds NW (ed) Evolution of crop plants. Longman, London, pp 273–277Google Scholar
  11. Grant V (1981) Plant speciation. Columbia University Press, New York, pp 1–563Google Scholar
  12. Gray JC, Kung SD, Wildman SG, Sheen SJ (1974) Origin of Nicotiana tabacum L. detected by polypeptide composition of Fraction I protein. Nature 252:226–227Google Scholar
  13. Huh TY, Gray MW (1982) Conservation of ribosomal RNA gene arrangement in the mitochondrial DNA of angiosperms. Plant Mol Biol 1:245–249Google Scholar
  14. Levings CS III (1983) The plant mitochondrial genome and its mutants. Cell 32:659–661Google Scholar
  15. Lonsdale DM, Hodge TP, Howe CJ, Stern DM (1983) Maize mitochondrial DNA contains a sequence homologous to the ribulose-1,5-bisphosphate carboxylase large subunit gene of chloroplast DNA. Cell 34:1007–1014Google Scholar
  16. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning (a laboratory manual). Cold Spring Harbor Laboratory, Cold Spring Harbor, pp 1–545Google Scholar
  17. Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–560Google Scholar
  18. Nagy F, Török I, Maliga P (1981) Extensive rearrangements in the mitochondrial DNA in somatic hybrids of Nicotiana tabacum and Nicotiana knightiana. Mol Gen Genet 183: 437–439Google Scholar
  19. Nagy F, Lázár G, Menczel L, Maliga P (1983) A heteroplasmic state induced by protoplast fusion is a necessary condition for detecting rearrangements in Nicotiana mitochondrial DNA. Theor Appl Genet 66:203–207Google Scholar
  20. Palmer JD (1982) Physical and gene mapping of chloroplast DNA from Atriplex triangularis and Cucumis sativa. Nucleic Acids Res 10: 1593–1605Google Scholar
  21. Sears BB (1980) Elimination of plastids during spermatogenesis and fertilization in the plant kingdom. Plasmid 4:233–255Google Scholar
  22. Sederoff RR, Levings CS III, Timothy DH, Hu WWL (1981) Evolution of DNA sequence organization in mitochondrial genomes of Zea. Proc Natl Acad Sci USA 78:5953–5957Google Scholar
  23. Sheen SJ (1972) Isozymic evidence bearing on the origin of Nicotiana tabacum L. Evolution 26:143–154Google Scholar
  24. Smith HH (1979) The genus as a genetic resource. In: Durbin RD (ed) Nicotiana: procedures for experimental use. US Dept Agric Techn Bull 1586, pp 1–16Google Scholar
  25. Sparks RB Jr, Dale RMK (1980) Characterization of 3Hlabeled supercoiled mitochondrial DNA from tobacco suspension culture cells. Mol Gen Genet 180:351–355Google Scholar
  26. Spruill WM, Levings CS III, Sederoff RR (1980) Recombinant DNA analysis indicates that the multiple chromosomes of maize mitochondria contain different sequences. Dev Genet 1:363–378Google Scholar
  27. Stern DB, Lonsdale DM (1982) Mitochondrial and chloroplast genomes of maize have a 12-kilobase DNA sequence in common. Nature 299:698–702Google Scholar
  28. Stern DB, Palmer JD (1984) Extensive and widespread homologies between mitochondrial DNA and chloroplast DNA in plants. Proc Natl Acad Sci USA 81:1946–1950Google Scholar
  29. Stern DB, Palmer JD, Thompson WF, Lonsdale DM (1983) Mitochondrial DNA sequence evolution and homology to chloroplast DNA in angiosperms. In: Goldberg RB (ed) Plant molecular biology. Alan R Liss, New York, pp 467–477Google Scholar
  30. Vieira J, Messing J (1982) The pUC plasmids, an M13mp7derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268Google Scholar
  31. Wahl GM, Stern M, Stark GR (1979) Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci USA 76:3683–3687Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • M. M. Bland
    • 1
  • D. F. Matzinger
    • 1
  • C. S. LevingsIII
    • 1
  1. 1.Department of GeneticsNorth Carolina State UniversityRaleighUSA

Personalised recommendations