Applied Microbiology and Biotechnology

, Volume 20, Issue 4, pp 275–277 | Cite as

Isolation of Robillarda sp. producing high β-1,4-glucan glucanohydrolase

  • Toshikazu Nagai
  • Yutaka Kashiwagi
  • Yumi Magae
  • Takashi Sasaki
Short Contribution
Received:

Summary

A fungus, Robillarda, sp. Y-20, which produces mainly, β-1,4-glucan glucanohydrolase (Cx-enzyme) in the culture filtrate, was isolated from soil. The specific activity of the Cx-enzyme was up to 4.9 times higher than that of Trichoderma reesei QM 9414.

Keywords

Glucan Trichoderma Culture Filtrate Trichoderma Reesei 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arx JA von (1974) The genera of fungi sporulating in pure culture. 2nd edn. J. Cramer, FRG, p. 145Google Scholar
  2. Ben-Ghedalia D, Miron J (1981) The effect of combined chemical and enzyme treatments on the saccharification and in vitro digestion rate of wheat straw. Biotechnol Bioeng 23: 823–831Google Scholar
  3. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275PubMedGoogle Scholar
  4. Mandels M, Ressei ET (1957) Induction of cellulase in Trichoderma viride as influenced by carbon sources and metals. J Bacteriol 73: 269–278Google Scholar
  5. Murao S, Kanamoto J, Arai M (1979) Isolation and identification of a cellulolytic enzyme producing microorganism. J Ferment Technol 57: 151–156Google Scholar
  6. Sasaki T, Tanaka T, Nanbu N, Sato Y, Kainuma K (1979) Correlation between X-ray diffraction measurements of cellulose crystalline structure and the susceptibility to microbial cellulase. Biotechnol Bioeng 21: 1031–1042Google Scholar
  7. Sasaki T, Sato Y, Nakagawa S, Shiraishi M, Kainuma K (1979) Crystallinity and enzymatic hydrolysis of cryomilled rice hull cellulose. J Jpn Soc Food Sci Technol 26: 523–529Google Scholar
  8. Somogyi M (1952) Notes on sugar determination. J Biol. Chem. 195: 19–23Google Scholar
  9. Wilke CR, Yang RD, Sciamanna AF, Freitas RP (1981) Raw materials evaluation and process development studies for conversion of biomass to sugars and ethanol. Biotechnol Bioeng 23: 163–183Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Toshikazu Nagai
    • 1
  • Yutaka Kashiwagi
    • 2
  • Yumi Magae
    • 2
  • Takashi Sasaki
    • 2
  1. 1.National Food Research InsitituteMinistry of Agriculture, Forestry and FisheriesTsukuba IbarakiJapan
  2. 2.Dai-Nippon Sugar Mfg. Co., Ltd.TokyoJapan

Personalised recommendations