Applied Microbiology and Biotechnology

, Volume 24, Issue 6, pp 477–486 | Cite as

Isolation and characterization of a cellulolytic actinomycete Microbispora bispora

  • C. R. WaldronJr.
  • C. A. Becker-Vallone
  • D. E. Eveleigh
Applied Microbiology


Protocols for the isolation of cellulolytic actinomycetes are described, and their use illustrated in the selection of thermophilic bacteria from soil. One isolate, Microbispora bispora, was selected for further study. It grew readily at 55°C, produced an extracellular cellulase in good yield (endoglucanase, 5.9 U/ml) that had a broad pH range (pH 5.5–7.2) and was thermally stable. Its aryl-β-glucosidase was cell-associated and was relatively resistant to end-product inhibition.


Cellulase Good Yield Thermophilic Bacterium Extracellular Cellulase Cellulolytic Actinomycete 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • C. R. WaldronJr.
    • 1
  • C. A. Becker-Vallone
    • 1
  • D. E. Eveleigh
    • 1
  1. 1.Department of Biochemistry and Microbiology, Cook CollegeRutgers UniversityNew BrunswickUSA

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