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Archives of Microbiology

, Volume 159, Issue 2, pp 114–118 | Cite as

Ultrastructure of Bacillus pulvifaciens

  • Jiri Ludvik
  • Oldrich Benada
  • Vera Drobniková
Original Papers

Abstract

The ultrastructure of vegetative cells and spores of Bacillus pulvifaciens was studied by CTEM and SEM methods. The vegetative cells are rods, 1.6–4.5 μm long and 0.4–0.6 μm wide, exhibiting typical ultrastructural features of Gram-positive bacteria. The spores are of ellipsoidal shape, 0.6×1.2 μm in size, with six longitudinal ribs reaching up to 130 nm in height. There are satelite ribs on both sides of the longitudinal ribs, reaching up to 20 nm in height. Between the longitudinal ribs, additional transversal ribs were observed in SEM. A special tubular layer, separating the outer and inner coat of the spores, was revealed in ultrathin sections. This layer seems to be a typical ultrastructural feature of Bacillus pulvifaciens spores.

Key words

Bacillus pulvifaciens Vegetative cells Spotes Ultrastructure Electron microscopy 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Jiri Ludvik
    • 1
  • Oldrich Benada
    • 1
  • Vera Drobniková
    • 2
  1. 1.Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4Czechoslovakia
  2. 2.Apiculture Research InstituteLibcice nad VltavouCzechoslovakia

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