Applied Microbiology and Biotechnology

, Volume 21, Issue 6, pp 341–347 | Cite as

Albomycin: Studies on fermentation, isolation and quantitative determination

  • H. -P. Fiedler
  • F. Walz
  • A. Döhle
  • H. Zähner


Streptomyces griseus TÜ 6 produces the sideromycin antibiotic albomycin δ2 in concentrations of approximately 1 mg/l. The production depends on the phosphate, iron, and ornithine concentrations in the medium. In optimized conditions, the production of albomycin could be increased to 25 mg/l in a fedbatch fermentation. Isolation and purification could be achieved by reversed-phase and size-exclusion chromatography and preparative high-performance liquid chromatography (HPLC). The detection limit in quantitative determination of albomycin by HPLC was reached at a concentration of 1 μg/ml, which was 100 times less sensitive than biological testing, but this method, although time-consuming, was more selective.


Iron Phosphate Chromatography Purification Fermentation 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • H. -P. Fiedler
    • 1
  • F. Walz
    • 1
  • A. Döhle
    • 1
  • H. Zähner
    • 1
  1. 1.Institut für Biologie II, Lehrstuhl Mikrobilogie ITübingenGermany

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