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Theoretical and Applied Genetics

, Volume 75, Issue 1, pp 132–137 | Cite as

Chromosome aberration and ploidy equilibrium of Vicia faba in tissue culture

  • D. L. Zhang
  • K. Q. Li
  • W. Gu
  • L. F. Hao
Originals

Summary

Different phytohormone concentrations induced different fequencies of various chromosome aberrations in calli of Vicia faba. NAA 10 ppm plus KT 2.5 ppm produced more haploids and NAA 30 ppm plus NAA 7.5 ppm produced more tetraploids and breakage. The relationship among the aberrations was analyzed. The hypothesis of ploidy equilibrium was established. The chromosome doubling rate and reduction rate of each treated group were calculated in relation to the observed data and the hypothesis. The frequency of tetraploids and breakage are correlated with each other. The frequency of total aberrations is linearly correlated with that of micronucleus formation. The regression equation is x=31.92+ 10.67 y.

Key words

Vicia faba Callus Chromosome aberration Somatic reduction Ploidy equilibrium 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • D. L. Zhang
    • 1
  • K. Q. Li
    • 1
  • W. Gu
    • 1
  • L. F. Hao
    • 1
  1. 1.Department of BiologyShaanxi Normal UniversityXian, ShaanxiChina

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