Chromosome aberration and ploidy equilibrium of Vicia faba in tissue culture
- 46 Downloads
- 7 Citations
Summary
Different phytohormone concentrations induced different fequencies of various chromosome aberrations in calli of Vicia faba. NAA 10 ppm plus KT 2.5 ppm produced more haploids and NAA 30 ppm plus NAA 7.5 ppm produced more tetraploids and breakage. The relationship among the aberrations was analyzed. The hypothesis of ploidy equilibrium was established. The chromosome doubling rate and reduction rate of each treated group were calculated in relation to the observed data and the hypothesis. The frequency of tetraploids and breakage are correlated with each other. The frequency of total aberrations is linearly correlated with that of micronucleus formation. The regression equation is x=31.92+ 10.67 y.
Key words
Vicia faba Callus Chromosome aberration Somatic reduction Ploidy equilibriumPreview
Unable to display preview. Download preview PDF.
References
- Cionini PG, Bennici A, D'Amato F (1978) Nuclear cytology of callus induction and development in vitro. I. Callus from Vicia faba cotyledons. Protoplasma 96:101–112Google Scholar
- D'Amato F, Bennici A, Cionini PG, Baroncelli S, Lupi MC (1980) Nuclear fragmentation followed by mitosis as mechanism for wide chromosome number variation in tissue culture: its implications for plant regeneration. In: Sala V, Parisi B, Cella R (eds) Plant cell culture: results and perspectives. Elsevier North-Holland Biochemical Press, pp 67–71Google Scholar
- Fang Z (1981) Report on the micronucleus test of plant cells for the detection of environmental pollution. J. Shandong Coll Oceanoll 11:1–5Google Scholar
- Hesemann CU (1980) Haploide Zellen in Kalli aus antheren Kallus von Vicia faba. Z. Pflanzenzücht 84:18–22Google Scholar
- Huskins CL (1948) Segregation and reduction in somatic tissues. J. Hered. 39:311–325Google Scholar
- Huskins CL, Cheng KC (1950) Segregation and reduction in somatic tissues: IV. Reductional groupings induced in Alliium cepa by low temperature. J. Hered 41:13–18Google Scholar
- Kunach VA (1984) Structural mutation in the population of plant tissue culture. Advances in modern genetics (in Russian). Science Press Moscow, pp 30–59Google Scholar
- Melchers G (1965) Einige genetische Gesichtspunkte zu sogenannten Gewebekulturen. Ber Dtsch. Bot Ges 78:21–29Google Scholar
- Mitra J, Steward FC (1961) Growth induction in cultures of Haplopappus gracilis: 2. The behavior of the nucleus. Am J Bot 48:358–368Google Scholar
- Orton TJ (1980) Chromosome variability in tissue cultures and regenerated plants of Hordeum. Theor Appl Genet 56:181–112Google Scholar
- Shen GP, Wang QN, Zhou ZZ (1985) Correlation between micronucleus and chromosome aberration. Hereditas 7:15–17Google Scholar
- Sunderland M (1976) Nuclear cytology. In: Street HE (ed) Plant tissue and cell culture. Oxford Press, London, pp 160–190Google Scholar
- Venketeswaran S (1963) Tissue culture studies of Vicia faba. 2. Cytology. Caryologia 16:91–100Google Scholar
- Yamane J (1975) Chromosomal variation in calluses induced in Vicia faba and Allilium cepa. Jpn J Genet 50:353–355Google Scholar