Theoretical and Applied Genetics

, Volume 75, Issue 1, pp 37–40 | Cite as

Isolation of tomato cell lines with altered response to Fusarium cell wall components

  • M. Buiatti
  • C. Simeti
  • S. Vannini
  • G. Marcheschi
  • A. Scala
  • P. Bettini
  • P. Bogani
  • M. G. Pellegrini
Originals

Summary

To obtain Tomato cell lines with an altered capacity to respond to heat-released cell wall components (elicitor) of a tomato pathogen (Fusarium oxysporum f. sp. lycopersici), positive and negative selection experiments, using BUdR enrichment techniques, were carried out on suspension cultures of the susceptible, low phytoalexin producer cultivar Red River. Both high and low phytoalexin producing clones were isolated. Further tests demonstrated that not all phytoalexin-producing clones were more susceptible to the elicitor toxic effect, and that they were altered also in the speed of response to fungal cell wall components. Cells selected with Fusarium elicitor showed the same behaviour when challenged by Phytophthora infestans elicitor, thus suggesting in this case lack of specificity. The results are finally discussed with a view to using the technique both as a tool to study the genetics and physiology of hostparasite interactions and as a possible new method for the selection of pathogen resistant genotypes.

Key words

Elicitor Fusarium oxysporum in vitro selection Pathogen resistance Phytoalexin 

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References

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • M. Buiatti
    • 1
  • C. Simeti
    • 1
  • S. Vannini
    • 1
  • G. Marcheschi
    • 1
  • A. Scala
    • 1
  • P. Bettini
    • 1
  • P. Bogani
    • 1
  • M. G. Pellegrini
    • 1
  1. 1.Department of Animal Biology and GeneticsFlorenceItaly

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