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Archives of Microbiology

, Volume 156, Issue 3, pp 176–180 | Cite as

Chemical characterization of the O-specific chain of Sphaerotilus natans ATCC 13338 lipopolysaccharide

  • Hussein Masoud
  • Andràs Neszmelyi
  • Hubert Mayer
Original Papers

Abstract

The chemical structure of the O-specific chain of the Sphaerotilus natans lipopolysaccharide was established. The isolated polysaccharide moiety contained neutral sugars (rhamnose, glucose, and l-glycero-d-manno-heptose), 3-deoxy-d-manno-octulosonic acid (Kdo), phosphate and ethanolamine. Alditol acetate and methylation analyses showed that the O-specific chain contained linear pentasaccharide repeating units, composed of four units of rhamnose, substituted at 3-position, and one unit of glucose, substituted at 4-position. Oxidation by chromium trioxide showed that all sugars were α-linked. The structure proposed for the O-specific chain has been confirmed by periodate oxidation, and by 1H-and 13C-NMR spectroscopic studies for the original and the Smith-degraded PS moiety. The O-specific unit of the S. natans LPS has the following structure:
$$\begin{gathered} [ \to 4) - Glup - (1\xrightarrow{a}3) - Rhap - (1\xrightarrow{a}3) - Rhap - \hfill \\ - (1\xrightarrow{a}3) - Rhap - (1\xrightarrow{a}3) - Rhap - (1]_n \xrightarrow{a} \hfill \\ \end{gathered} $$

Key words

Sphaerotilus natans Lipopolysaccharide O-specific chain DOC-PAGE Methylation analysis 

Abbreviations

LPS

lipopolysaccharide

PS

polysaccharide

Kdo

3-deoxy-d-manno-octulosonic acid

ld

heptose, l-glycero-d-manno-heptose

NMR

nuclear magnetic resonance

APT

attached proton test

GC-MS

gas-chromatography/mass spectrometry

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Hussein Masoud
    • 1
  • Andràs Neszmelyi
    • 2
  • Hubert Mayer
    • 1
  1. 1.Max-Planck-Institut für ImmunbiologieFreiburgGermany
  2. 2.Central Research Institute for Chemistry of the Hungarian Academy of SciencesBudapestHungary

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