Archives of Microbiology

, Volume 156, Issue 3, pp 167–175 | Cite as

The structure of the lipid A component of Sphaerotilus natans

  • Hussein Masoud
  • Teresa Urbanik-Sypniewska
  • Buko Lindner
  • Jürgen Weckesser
  • Hubert Mayer
Original Papers


The lipopolysaccharide of Sphaerotilus natans afforded a ladder-like pattern of bands in sodium deoxycholate-polyacrylamide gel electrophoresis, indicating the presence of a S-form lipopolysaccharide. The chemical analysis showed neutral sugars (rhamnose, glucose, l-glycero-d-manno-heptose), 3-deoxy-octulosonic acid (Kdo), amino compounds (glucosamine, glucosamine phosphate, ethanolamine and ethanolamine phosphate), and phosphorus. The lipid A fraction contained saturated and unsaturated capric, lauric, and myristic acids, and 3-hydroxy capric acid (3-OH-10:0). Its chemical structure was consisting of a glucosamine disaccharide, glycosidically substituted by a phosphomonoester, and substituted at C-4′ by a pyrophosphodiester esterified with ethanolamine. The amino groups of both glucosamines are acylated by 3-hydroxy capric acids and these in turn are substituted by saturated and unsaturated capric, lauric, and myristic acids. Hydroxyl groups of the backbone disaccharide at C-3 and C-3′ were also esterified by 3-hydroxy capric acid, those at C-4 and C-6 were unsubstituted. The latter provides the attachment site for Kdo.

Key words

Sphaerotilus natans Lipopolysaccharide Lipid A Laser desorption mass spectrometry DOC-PAGE 3-Hydroxycapric acid Proteobacteria 



3-deoxy-d-manno-octulosonic acid


3-hydroxy capric acid


deoxycholate-polyacrylamide gel electrophoresis


gas chromatography/mass spectrometry


laser desorption mass spectrometry






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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Hussein Masoud
    • 1
  • Teresa Urbanik-Sypniewska
    • 1
  • Buko Lindner
    • 2
  • Jürgen Weckesser
    • 3
  • Hubert Mayer
    • 1
  1. 1.Max-Planck-Institut für ImmunbiologieFreiburgGermany
  2. 2.Forschungsinstitut BorstelBorstelGermany
  3. 3.Institut für Biologie II, MikrobiologieAlbert-Ludwigs-Universität FreiburgFreiburgGermany

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