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Archives of Microbiology

, Volume 153, Issue 3, pp 276–281 | Cite as

Rhizocticin A, an antifungal phosphono-oligopeptide of Bacillus subtilis ATCC 6633: biological properties

  • Martin Kugler
  • Wolfgang Loeffler
  • Claudius Rapp
  • Armin Kern
  • Günther Jung
Original Papers

Abstract

Rhizocticin A, the main component of the antifungal, hydrophilic phosphono-oligopeptides of Bacillus subtilis ATCC 6633, was used for sensitivity testing and experiments into the molecular mechanism of the antibiotic action. Budding and filamentous fungi as well as the cultivated nematode Caenorhabditis elegans were found to be sensitive, whereas bacteria and the protozoon Paramecium caudatum were insensitive. Rhizoctonia solani was inhibited in agar dilution tests but not in diffusion tests. The antifungal effect of rhizocticin A was neutralized by a variety of amino acids and oligopeptides. Oligopeptide influence was mainly understood as transport antagonism, and it was concluded that the antibiotic enters the recipeint cell via the peptide transport system. l- and d-cystine were also identified as potent, general antagonists of the oligopeptide transport. The rhizocticin-antagonism of four other amino acids was taken as a clue to the site of action. Provided that rhizocticin A is split by peptidases of the target cell into inactive l-arginine and toxic l-2-amino-5-phosphono-3-cis-pentenoic acid (l-APPA), the latter may interfere with the threonine or threonine-related metabolism.

Key words

Rhizocticins Phosphono-oligopeptide antibiotics Bacillus subtilis Antifungal spectrum Rhizocticin uptake Transport antagonism Mechanism of action 

Abbreviations

APPA

(2-amino-5-phosphono-3-cis-pentenoic acid)

B.

(Bacillus)

P.

(Paecilomyces)

S.

(Saccharomyces)

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Martin Kugler
    • 1
  • Wolfgang Loeffler
    • 1
  • Claudius Rapp
    • 2
  • Armin Kern
    • 2
  • Günther Jung
    • 2
  1. 1.Institut für Mikrobiologie I der Universität TübingenTübingenFederal Republic of Germany
  2. 2.Institut für Organische Chemie der Universität TübingenTübingenFederal Republic of Germany

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