Archives of Microbiology

, Volume 158, Issue 1, pp 68–73 | Cite as

An Escherichia coli mutant containing only demethylmenaquinone, but no menaquinone: effects on fumarate, dimethylsulfoxide, trimethylamine N-oxide and nitrate respiration

  • U. Wissenbach
  • D. Ternes
  • G. Unden
Original Papers


The mutant strain AN70 (ubiE) of Escherichia coli which is known to lack ubiquinone (Young IG et al. 1971), was analyzed for menaquinone (MK) and demethylmenaquinone (DMK) contents. In contrast to the wild-type, strain AN70 contained only DMK, but no MK. The mutant strain was able to grow with fumarate, trimethylamine N-oxide (TMAO) and dimethylsulfoxide (DMSO), but not with nitrate as electron acceptor. The membranes catalyzed anaerobic respiration with fumarate and TMAO at 69 and 74% of wild-type rates. DMSO respiration was reduced to 38% of wild-type activities and nitrate respiration was missing (≦8% of wild-type), although the respective enzymes were present in wild-type rates. The results complement earlier findings which demonstrated a role for DMK only in TMAO respiration (Wissenbach et al. 1990). It is concluded, that DMK (in addition to MK) can serve as a redox mediator in fumarate, TMAO and to some extent in DMSO respiration, but not in nitrate respiration. In strain AN70 (ubiE) the lack of ubiquinone (Q) is due to a defect in a specific methylation step of Q biosynthesis. Synthesis of MK from DMK appears to depend on the same gene (ubiE).

Key words

Demethylmenaquinone Menaquinone Anacrobic respiration Fumarate respiration Nitrate respiration Escherichia coli 







trimethylamine N-oxide



BV =


BVred =

reduced benzylyiologen

Q =


MK =




NQ =



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  1. Bentley R, Meganathan R (1987) Biosynthesis of the isoprenoid quinones ubiquinone and menaquinone. In: Neidhardt FC (ed) Escherichia coli and Salmonella typhimurium. Cellular and molecular biology. American Soc Microbiol., Washington DC, pp 512–520Google Scholar
  2. Bode CH, Goebell H, Stähler E (1968) Zur Eliminierung von Trübungsfehlern bei der Eiweißbestimmung mit der Biuretmethode. Z Klin Chem Biochem 5: 419–422Google Scholar
  3. Guest JR (1977) Menaquinone biosynthesis: mutants of Escherichia coli K-12 requiring 2-succinylbenzoate. J Bacteriol 130: 1038–1046Google Scholar
  4. Guest JR (1979) Anaerobic growth of Escherichia coli K12 with fumarate as terminal electron acceptor. Genetic studies with menaquinone and fluoro-acetate-resistent mutants. J Gen Microbiol 115: 259–271Google Scholar
  5. Holländer R (1976) Correlation of the function of demethyl-menaquinone in bacterial electron transport with its redox potentia. FEBS Lett 72: 98–100Google Scholar
  6. Kröger A, Dadák (1969) On the role of quinones in bacterial electron transport. Eur J Biochem 11: 328–340Google Scholar
  7. Kröger A, Dadák V, Klingenberg M, Diemer F (1971) On the role of quinones in bacterial electron transport: differential roles of ubiquinone and menaquinone in Proteus rettgert. Eur J Biochem 21: 322–333Google Scholar
  8. Kroppenstedt RM (1985) Fatty acid and menaquinone analysis of Actinomycetes and related organisms. In: Goodfellow M, Minnikin E (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 173–199Google Scholar
  9. Kroppenstedt RM, Mannheim W (1989) Lipoquinones in members of the family Pasteurellaceae. Int J Syst Bacteriol 39: 304–308Google Scholar
  10. Kwan HS, Barrett EL (1983) Roles for menaquinone and the two trimethylamine oxide (TMAO) reductases in TMAO respiration in Salmonella typhimurium: Mu d(Apr lac) insertion mutations in men and tor. J Bacteriol 155: 1147–1155Google Scholar
  11. Mannheim W, Stieler W, Wolf G, Zabel R (1978) Taxonomic significance of respiratory quinones and fumarate respiration in Actinobacillus and Pasteurella. Int J Syst Bacteriol 28: 7–13Google Scholar
  12. Meganathan R (1984) Inability of men mutants of Escherichia coli to use trimethylamine-N-oxide as an electron acceptor. FEMS Microbiol Letters 24: 57–62Google Scholar
  13. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  14. Schnorf U (1966) Der Eifluß von Substituenten auf Redoxpotential und Wuchseigenschaften von Chinonen. Doctoral thesis, No. 3871, ETH Zürich, Faculty of ChemistryGoogle Scholar
  15. Stroobant P, Young IG, Gibson F (1972) Mutants of Escherichia coli K-12 blocked in the final reaction of ubiquinone biosynthesis: characterization and genetic analysis. J Bacteriol 109: 134–139Google Scholar
  16. Unden G (1988) Differential roles for menaquinone and demethyl-menaquinone in anaerobic electron transport of E. coli and their fnr-dependent expression. Arch Microbiol 150: 499–503Google Scholar
  17. UndeN G, Böcher R, Knecht J, Kröger A (1982) Hydrogenase from Vibrio succinogenes, a nickel protein. FEBS Lett 145: 230–234Google Scholar
  18. Wallace BJ, Young IG (1977a) Aerobic respiration in mutants of Escherichia coli accumulating quinone analogues of ubiquinone. Biochim Biophys Acta 461: 75–83Google Scholar
  19. Wallace BJ, Young IG (1977b) Role of quinones in electron transport to oxygen and nitrate in Escherichia coli. Biochim Biophys Acta 461: 84–100Google Scholar
  20. Whistance GR, Threlfall DR (1968) Effect of anaerobiosis on the concentration of demethylmenaquinone, menaquinone and ubiquinone in Escherichia freundii, Proteus mirabilis and Aeromonas punctata. Biochem J 108: 505–507Google Scholar
  21. Wissenbach U, Kröger A, Unden G (1990) The specific functions of menaquinone and demethylmenaquinone in anaerobic respiration with fumarate, dimethylsulfoxide, trimethylamine N-oxide and nitrate by Escherichia coli. Arch Microbiol 154: 60–66Google Scholar
  22. Young IG, McCann LM, Stroobant P, Gibson F (1971) Characterization and genetic analysis of mutant strains of Escherichia coli K-12 accumulating the ubiquinone precursors 2-octaprenyl-6-methoxy-1,4-benzoquinone and 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone. J Bacteriol 105: 769–778Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • U. Wissenbach
    • 1
  • D. Ternes
    • 1
  • G. Unden
    • 1
  1. 1.Institut für BiochemieHeinrich Heine-UniversitätDüsseldorfGermany

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