Archives of Microbiology

, Volume 159, Issue 6, pp 541–544 | Cite as

Endogenous respiration reflects the energy load imposed by transport of nonmetabolizable substrates and by induced de novo protein synthesis in Rhodotorula glutinis

  • S. Janda
  • K. Sigler
  • M. Höfer
Original Papers


Uptake of the nonmetabolizable sugars 6-deoxy-d-glucose, l-rhamnose and l-xylose, which are taken up by a common carrier, stimulated significantly cell respiration in Rhodotorula glutinis. The extra oxygen consumption for uptake (0.5–0.7 equivalents O2/mol transported sugar) was proportional to the uptake rate and was independent of the Ktvalue of the transport system. Sugars that become metabolized after induction, d-arabinose and methyl-α-d-glucoside, caused a higher stimulation, 1.4 and 3.6 equivalents O2/mol respectively, which was reduced to 0.6 equivalents O2/mol when de novo protein synthesis was blocked by cycloheximide. The stimulation of respiration thus includes a fraction related purely to the energy demand for uptake and another one related to the induced de novo protein synthesis. The net uptake-induced respiration boost was similar with all sugars under study irrespective of their transport systems. The estimated energy demand was equivalent to about 2 ATP/sugar molecule. For comparison, the amino acid analogue α-aminoisobutyric acid (AIB) was also investigated; the overall energy demand for its uptake corresponded to the equivalent of about 4 ATP/molecule.

Key words

Substrate uptake Energy requirement Endogenous respiration Nonmetabolized substrate Induced catabolism Rhodotorula glutinis 



α-aminoisobutyric acid


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Decker M, Tanner W (1972) Respiratory increase and active hexose uptake of Chlorella vulgaris. Biochim Biophys Acta 266: 661–669CrossRefGoogle Scholar
  2. Gille G, Höfer M, Sigler K (1992) Evidence for a specific fructose carrier in Rhodotorula glutinis based on kinetic studies with a mutant defective in glucose transport. Folia Microbiol (Praha) 37: 31–38CrossRefGoogle Scholar
  3. Grüneberg A, Komor E (1976) Different proton-sugar stoichiometries for the uptake of glucose analogues by Chlorella vulgaris. Evidence for sugar-dependent proton uptake without concomitant sugar uptake by the proton-sugar symport system. Biochim Biophys Acta 448: 133–142CrossRefGoogle Scholar
  4. Hauer R, Höfer M (1978) Evidence for interaction between the energy-dependent transport of sugars and the membrane potential in the yeast Rhodotorula gracilis (Rhodosporidium toruloides). J Membr Biol 43: 335–349CrossRefGoogle Scholar
  5. Höfer M (1970) Mobile membrane carrier for monosaccharide transport in Rhodotorula gracilis. J Membr Biol 3: 73–82CrossRefGoogle Scholar
  6. Höfer M (1989) Accumulation of electroneutral and charged carbohydrates by proton cotransport in Rhodotorula. Methods Enzymol 174: 629–653CrossRefGoogle Scholar
  7. Höfer M, Kotyk A (1968) Tight coupling of monosaccharide transport and metabolism in Rhodotorula gracilis. Folia Microbiol (Praha) 13: 197–204CrossRefGoogle Scholar
  8. Höfer M, Misra PC (1978) Evidence for a proton/sugar symport in the yeast Rhodotorula gracilis (glutinis). Biochem J 172: 15–22CrossRefGoogle Scholar
  9. Höfer M, Betz A, Kotyk A (1971) Metabolism of the obligatory aerobic yeast Rhodotorula gracilis. IV. Induction of an enzyme necessary for d-xylose catabolism. Biochim Biophys Acta 252: 1–12CrossRefGoogle Scholar
  10. Janda S (1976) Transport interactions among sugars in Rhodotorula glutinis. Wiss Z Humboldt-Univ Berlin, Math-Naturwiss R 25: 115–116Google Scholar
  11. Janda S (1979) Relationship of active membrane transport and respiration in Rhodotorula glutinis: possibility of two respiratory systems. Cell Mol Biol 25: 131–136Google Scholar
  12. Janda S, Sigler K (1987) Uptake of nonmetabolized substrate analogues in the yeast Rhodotorula glutinis. Proceedings of the 5th Small Meeting on Yeast Transport and Energetics, p 12. L'Université Catholique de Louvain, Louvain-la-NeuveGoogle Scholar
  13. Janda S, Tauchová R (1982) Cyanide and antimycin A resistant respiration and uptake of 6-deoxy-d-glucose in Rhodotorula glutinis. Cell Mol Biol 28: 547–553PubMedGoogle Scholar
  14. Janda S, Beneš I, Opekarová M, Št'astná J, Tauchová R (1990) Effect of hydrogen peroxide on the aerobic yeast Rhodotorula glutinis. Microbios Lett 43: 37–42Google Scholar
  15. Kotyk A, Höfer M (1965) Uphill transport of sugars in the yeast Rhodotorula gracilis. Biochim Biophys Acta 102: 410–420CrossRefGoogle Scholar
  16. Saiyd NH, Kotyk A (1971) Utilization of amino acids by Rhodotorula glutinis. Folia Microbiol (Praha) 16: 438–444Google Scholar
  17. Serrano R (1988) Structure and function of proton translocating ATPase in plasma membranes of plants and fungi. Biochim Biophys Acta 947: 1–28CrossRefGoogle Scholar
  18. Sigler K, Höfer M (1991) Mechanisms of acid extrusion in yeast. Biochim Biophys Acta 1071: 375–391CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • S. Janda
    • 1
  • K. Sigler
    • 1
  • M. Höfer
    • 2
  1. 1.Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4Czech Republic
  2. 2.Bolanisches Institut der Universität BonnBonn 1Germany

Personalised recommendations