Archives of Microbiology

, Volume 154, Issue 6, pp 594–599 | Cite as

Isolate B12, which harbours a virus-like element, represents a new species of the archaebacterial genus Sulfolobus, Sulfolobus shibatae, sp. nov.

  • Dennis Grogan
  • Peter Palm
  • Wolfram Zillig
Original Papers


The Sulfolobus isolate B12 and its endogenous virus-like element SSV1 have provided a fruitful system for detailed analysis of certain aspects of archaebacterial molecular biology, especially those concerning gene expression. In the course of clarifying this isolate's taxonomic position, we determined DNA base composition, ability to grow autotrophically, nucleotide sequence of 16S ribosomal RNA, and level of total genomic homology to other Sulfolobus strains. Although the results generally demonstrate a similarity to S. solfataricus, DNA-DNA hybridisation and 16S rRNA sequence data indicate that isolate B12 in fact represents a distinct species.

Key words

Sulfolobus isolate B12 Archaebacterial taxonomy G+C content Sulphur oxidation DNA-DNA hybridisation 16S rRNA sequence 



Deutsche Sammlung von Mikroorganismen, Mascheroder Weg 1 B, D-3300 Braunschweig, FRG


sodium dodecyl sulphate-polyacrylamide gel electrophoresis


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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Dennis Grogan
    • 1
  • Peter Palm
    • 1
  • Wolfram Zillig
    • 1
  1. 1.Max-Planck-Institut für BiochemieMartinsriedFederal Republic of Germany

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