Archives of Microbiology

, Volume 156, Issue 5, pp 344–349 | Cite as

Cytological peculiarities of some extremely halophilic soil archaeobacteria

  • N. A. Kostrikina
  • I. S. Zvyagintseva
  • V. I. Duda
Original Papers


Ultrastructure and morphogenesis of extremely halophilic neutrophilic (Halobacteriam distributum, Halococcus turkmenicus) and alkaliphilic (Natronobacterium pharaonis, Natronococcus occultus) archaeobacteria were studied. The H. distributum culture was rather polymorphous and produced cells of four types. Due to the irregular cell fission in different planes packages of various numbers of cells surrounded by a common capsule were formed. Resting forms (halocysts) with multilayer covers were present in the population. The N. pharaonis culture consisted of rod-like cells and cyst-like forms. Besides, under conditions of carbon limitation, multicellular aggregated forms were found in the culture. Encapsulated single cells and aggregated forms with a common capsule were observed in H. turkmenicus and N. occultus cultures.

Key words

Archaeobacteria Halobacterium distributum Halococcus turkmenicus Natronobacterium pharaonis Natronococcus occultus Halocysts 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Chumakov KM, Zhilina TN, Zvyagintseva IS, Tarasov AL, Zavarzin GA (1987) 5S rRNA in Archaeobacteria. J Gen Biol 48: 167–181 (in Russian)Google Scholar
  2. Glauert AM, Thornley M (1966) Glutaraldehide fixation of Gramnegative bacteria. J R Microsc Soc 85: 449–455Google Scholar
  3. Ivanova H, Zvyagintseva IS, Tarasov AL, Lysogorskaya EA (1989) Exocellular enzymes of extreme halophilic archaeobacteria. Microbiologia 58: 251–255 (in Russian)Google Scholar
  4. Ishiguro R, Wolfe RS (1970) Control of morphogenesis in Geodermatophilus: ultrastructural studies. J Bacteriol 104: 566–580Google Scholar
  5. Kellenberger E, Ryter A, Sechaud Y (1958) Electron microscope study of DNA-containing plasma. J Biophys Biochem Cytol 4: 671–676Google Scholar
  6. Kessel M, Cohen Y (1982) Ultrastructure of square bacteria from a brine pool in soulthern Sinai. J Bacteriol 150: 851–860Google Scholar
  7. Kevbrina MV, Zvyagintseva IS, Plakunov VK (1989) Natronococcus occultus 3A growth in media with acetate. Microbiologia 58: 377–381 (in Russian)Google Scholar
  8. Kushner DJ (1981) Life of microorganisms under high salts and dilute compounds: halophilic bacteria. In: Kushner DJ (ed) Microbial life in extreme environments. Mir, Miscow (English Translation) pp 365–425Google Scholar
  9. Larsen H (1962) Halophilism. In: Woese CR, Wolfe RS (eds) The bacteria. Academic Press, New York, L. 4, pp 297–342Google Scholar
  10. Marshall KC (1976) Interfaces in microbial ecology. Harvard Univ Press, p 156Google Scholar
  11. Robertson JD, Schreil W, Reedy M (1982) Halobacterium halobium 1. A thin-sectioning electron-microscopic study. J Ultrastruct Res 80: 148–162Google Scholar
  12. Robinson RW (1986) Life cycles in the methanogenic archaebacterium Methanosarcina mazei. Appl Environ Microbiol 52: 17–27Google Scholar
  13. Robinson R, Aldrich HC, Hurst SF, Bleiweis AS (1985) The role of the cell surface of Methanosarcina mazei in cell aggregation. Appl Environ Microbiol 49: 321–327Google Scholar
  14. Rodriquez-Valera F, Juez G, Kushner DJ (1983) Halobacterium mediterranei sp nov, a new carbohydrate-utilizing extreme halophile. Syst Appl Microbiol 4: 369–381Google Scholar
  15. Tindall BJ, Mills AA, Grant WD (1980) An alkaliphilic red halophilic bacterium with a low magnesium requirement from a Keyan soda lake. J Gen Microbiol 116: 257–260Google Scholar
  16. Tindall BJ, Ross HNM, Grant WD (1984) Natronobacterium gen. nov. and Natronococcus gen. nov., two new genera of haloalkaliphilic archaeobacteria. Syst Appl Microbiol 5: 41–57Google Scholar
  17. Tomlinson GA, Hochstein LG (1972) Studies on acid production during carbohydrate metabolism by extremely halophilic bacteria. Can J Microbiol 18: 1973–1976Google Scholar
  18. Wais AC (1985) Cellular morphogenesis in a halophilic archaeobacterium. Curr Microbiol 12: 191–196Google Scholar
  19. Zhilina TN, Zavarzin GA (1979) Comparative cytology of Methanosarcina and description of Methanosarcina vacuolata n. sp. Microbiologia 48: 279–285 (in Russian)Google Scholar
  20. Zvyagintsev DG (1987) Soil and Microorganisms. Moscow State University Publishing House, p 256 (in Russian)Google Scholar
  21. Zvyagintseva IS, Tarasov AL (1987) Extreme halophilic bacteria from saline soils. Microbiologia 56: 639–844 (in Russian)Google Scholar
  22. Zvyagintseva IS, Tarasov AL, Kostrikina NA (1988) Extreme halophilic and haloalkaliphilic archaeobacteria. In: Zavarzin GA (ed) Archaeobacteria. Scientific Centre of Biological Investigations, Puschino, pp 67–76 (in Russian)Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • N. A. Kostrikina
    • 1
  • I. S. Zvyagintseva
    • 1
  • V. I. Duda
    • 1
  1. 1.Institute of Microbiology of the USSR Science AcademyMoscowUSSR

Personalised recommendations