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Archives of Microbiology

, Volume 155, Issue 2, pp 164–169 | Cite as

Molybdenum-dependent degradation of quinoline by Pseudomonas putida Chin IK and other aerobic bacteria

  • Martina Blaschke
  • Annette Kretzer
  • Christine Schäfer
  • Matthias Nagel
  • Jan R. Andreesen
Original Papers

Abstract

Eighteen different aerobic bacteria were isolated which utilized quinoline as sole source of carbon, nitrogen, and energy. Attempts were unsuccessful at isolating anaerobic quinoline-degrading bacteria. The optimal concentration of quinoline for growth was in the range of 2.5 to 5 mM. Some organisms excreted 2-hydroxyquinoline as the first intermediate. Hydroxylation of quinoline was catalyzed by a dehydrogenase which was induced in the presence of quinoline or 2-hydroxyquinoline. Quinoline dehydrogenase activity was dependent on the availability of molybdate in the growth medium. Growth on quinoline was inhibited by tungstate, an antagonist of molybdate. Partially purified quinoline dehydrogenase from Pseudomonas putida Chin IK indicated the presence of flavin, iron-sulfur centers, and molybdenum-binding pterin. Mr of quinoline dehydrogenase was about 300 kDa in all isolates investigated.

Key words

2-Hydroxyquinoline Molybdenum-binding pterin Quinoline Quinoline dehydrogenase 

Abbreviations

APS

ammonium peroxodisulfate

DCPIP

2,6-dichlorophenol-indophenol

EEO

electroendosmosis

MTT

thiazolyl blue

PES

phenazine ethosulfate

TEMED

N,N,N′,N′-tetramethyl-ethylenediamine

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Martina Blaschke
    • 1
  • Annette Kretzer
    • 1
  • Christine Schäfer
    • 1
  • Matthias Nagel
    • 1
  • Jan R. Andreesen
    • 1
  1. 1.Institut für Mikrobiologie der UniversitätGöttingenGermany

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