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Archives of Microbiology

, Volume 155, Issue 2, pp 159–163 | Cite as

Regulation of methanol oxidation and carbon dioxide fixation in Xanthobacter strain 25a grown in continuous culture

  • L. M. Croes
  • W. G. Meijer
  • L. Dijkhuizen
Original Papers

Abstract

The regulation of C1-metabolism in Xanthobacter strain 25a was studied during growth of the organism on acetate, formate and methanol in chemostat cultures. No activity of methanol dehydrogenase (MDH), formate dehydrogenase (FDS) or ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisC/O) could be detected in cells grown on acetate alone over a range of dilution rates tested. Addition of methanol or formate to the feed resulted in the immediate induction of MDH and FDH and complete utilization (D=0.10 h-1) of acetate and the C1-substrates. The activities of these enzymes rapidly dropped at the higher growth rates, which suggests that their synthesis is further controlled via repression by “heterotrophic” substrates such as acetate. Synthesis of RuBisC/O already occurred at low methanol concentrations in the feed, resulting in additive growth yields on acetate/methanol mixtures. The energy generated in the oxidation of formate initially allowed an increased assimilation of acetate (and a decreased dissimilation), resulting in enhanced growth yields on the mixture. RuBisC/O activity could only be detected at the higher formate/acetate ratios in the feed. The data suggest that synthesis of RuBisC/O and CO2 fixation via the Calvin cycle in Xanthobacter strain 25 a is controlled via a (de)repression mechanism, as is the case in other facultatively autotrophic bacteria. Autotrophic CO2 fixation only occurs under conditions with a diminished supply of “heterotrophic” carbon sources and a sufficiently high availability of suitable energy sources. The latter point is further supported by the clearly more pronounced derepressing effect exerted by methanol compared to formate.

Key words

Xanthobacter Methanol Formate Methylotrophy Autotrophy Calvin cycle Regulation Continuous culture RuBisC/O Carbon dioxide fixation 

Abbreviations

FDH

formate dehydrogenase

FBPase

fructose-1,6-bisphosphatase

ICDH

isocitrate dehydrogenase

MDH

methanol dehydrogenase

PQQ

pyrrolo quinoline quinone

PRK

phosphoribulokinase

RuBisC/O

ribulose-1,5-bisphosphate carboxylase/oxygenase

RuMP

ribulose monophosphate

TCA

tricarboxylic acid cycle

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • L. M. Croes
    • 1
  • W. G. Meijer
    • 1
  • L. Dijkhuizen
    • 1
  1. 1.Department of MicrobiologyUniversity of GroningenHarenThe Netherlands

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