Archives of Microbiology

, Volume 155, Issue 2, pp 149–152 | Cite as

Inhibition of aconitase and fumarase by nitrogen compounds in Rhodobacter capsulatus

  • M. Martinez Luque-Romero
  • F. Castillo
Original Papers


High levels of aconitase and fumarase activities were found in Rhodobacter capsulatus E1F1 cells cultured with nitrate as the sole nitrogen source either under light-anaerobic or dark-aerobic conditions. Both activities were strongly and reversibly inhibited in vitro by nitrite or nitric oxide, whereas nitrate or hydroxylamine showed a lower effect. Other enzymes of the tricarboxylic acids cycle such as malate dehydrogenase or isocitrate dehydrogenase were not affected by these nitrogen compounds. When growing on nitrate in the dark R. capsulatus E1F1 cells accumulated nitrite intracellularly, so that an in vivo inhibition of aconitase and fumarase could account for the strong inhibition of growth observed in the presence of nitrite under dark-aerobic conditions.

Key words

Nitrate photoassimilation Nitrite Fumarase Aconitase Rhodobacter capsulatus 







malate dehydrogenase


isocitrate dehydrogenase


tricarboxylic acid


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • M. Martinez Luque-Romero
    • 1
  • F. Castillo
    • 1
  1. 1.Departamento de Bioquímica y Biología Molecular y FisiologíaFacultad de CienciasCórdobaSpain

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