Archives of Microbiology

, Volume 159, Issue 3, pp 282–288 | Cite as

Isolation and characterization of a new spore-forming sulfate-reducing bacterium growing by complete oxidation of catechol

  • Jan Kuever
  • Juergen Kulmer
  • Sigrid Jannsen
  • Ulrich Fischer
  • Karl-Heinz Blotevogel
Original Papers


A new mesophilic sulfate-reducing bacterium, strain Groll, was isolated from a benzoate enrichment culture inoculated with black mud from a freshwater ditch. The isolate was a spore-forming, rod-shaped, motile, gram-positive bacterium. This isolate was able of complete oxidation of several aromatic compounds including phenol, catechol, benzoate, p-and m-cresol, benzyl alcohol and vanillate. With hydrogen and carbon dioxide, formate or O-methylated aromatic compounds, autotrophic growth during sulfate reduction or homoacetogenesis was demonstrated. Lactate was not used as a substrate. SO inf4 sup2- , SO inf3 sup2- , and S2O inf3 sup2- were utilized as electron acceptors. Although strain Groll originated from a freshwater habitat, salt concentrations of up to 30 g·l-1 were tolerated. The optimum temperature for growth was 35–37°C. The G+C content of DNA was 42.1 mol%. This isolate is described as a new species of the genus Desulfotomaculum.

Key words

Sulfate reduction Desulfotomaculum Anaerobic catechol oxidation Degradation and transformation of aromatic compounds 


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  1. Bache R, Pfennig N (1981) Selective isolation of Acetobacterium woodii on methoxylated aromatic acids and determination of growth yields. Arch Microbiol 130: 255–262Google Scholar
  2. Bak F, Widdel F (1986) Anaerobic degradation of phenol and phenol derivatives by Desulfobacterium phenolicum sp. nov. Arch Microbiol 146: 177–180Google Scholar
  3. Balba MT, Evans WC (1980) The methanogenic biodegradation of catechol by a microbial consortium: evidence for the production of phenol through cis-benzenediol. Biochem Soc Trans 8: 452–453Google Scholar
  4. Bradford MM, (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254Google Scholar
  5. Cline E (1969) Spectrophotometric determination of hydrogen-sulfide in natural waters. Limnol Oceanogr 14: 454–458Google Scholar
  6. Cord-Ruwisch R, Garcia JL (1985) Isolation and characterization of an anaerobic benzoate-degrading spore-forming sulfate-reducing bacterium, Desulfotomaculum sapomandens sp. nov. FEMS Microbiol Lett 29: 325–330Google Scholar
  7. Cypionka H, Pfennig N (1986) Growth yields of Desulfotomaculum orientis with hydrogen in chemostat culture. Arch Microbiol 143: 396–399Google Scholar
  8. DeWeerd KA, Mandelco L, Tanner RS, Woese CR, Suflita JM (1990) Desulfomonile tiedejei gen nov. and sp. nov., a novel anaerobic, dehalogenating, sulfate-reducing bacterium. Arch Microbiol 154: 23–30Google Scholar
  9. Evans WC, Fuchs G (1988) Anaerobic degradation of aromatic compounds. Ann Rev Microbiol 42: 289–317Google Scholar
  10. Healy JB, Young LY (1980) Anaerobic degradation of eleven aromatic compounds to methane. Appl Environ Microbiol 38: 84–89Google Scholar
  11. Kaiser J-P, Hanselmann KW (1982) Fermentative metabolism of substituted mono-aromatic compounds by a bacterial community from anaerobic sediments. Arch Microbiol 133: 185–194Google Scholar
  12. Lux MF, Keith E, Hsu T, Drake HL (1990) Biotransformation of aromatic aldehydes by acetogenic bacteria. FEMS Microbiol Lett 67: 73–78Google Scholar
  13. Marmur J, Doty P (1962) Determination of the base composition of desoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118Google Scholar
  14. Pfennig N, Widdel F, Trüper HG (1981) The dissimilatory sulfate-reducing bacteria. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) the prokaryotes, vol 1. Springer, New York Berlin Heidelberg, pp 926–944Google Scholar
  15. Postgate JR (1956) Cytochrome C3 and desulfoviridin: pigments of the anaerobic Desulphovibrio desulphuricans. J Gen Microbiol 14: 545–572Google Scholar
  16. Schauder R, Eikmanns B, Thauer RK, Widdel F, Fuchs G (1986) Acetate oxidation to CO2 in anaerobic bacteria via a novel pathway not involving reactions of the citric acid cycle. Arch Microbiol 145: 162–172Google Scholar
  17. Schnell S, Bak F, Pfenning N (1989) Anaerobic degradation of aniline and dihydroxybenzenes by newly isolated sulfate-reducing bacteria and description of Desulfobacterium anilini. Arch Microbiol 152: 556–563Google Scholar
  18. Szewzyk R, Pfennig N (1987) Complete oxidation of catechol by strictly anaerobic sulfate-reducing Desulfobacterium catecholicum sp. nov. Arch Microbiol 147: 163–168Google Scholar
  19. Tasaki M, Kamagata Y, Nakamura K, Mikami E (1991) Isolation and characterization of a thermophilic benzoate-degrading, sulfate-reducing bacterium, Desulfotomaculum thermobenzoicum sp. nov. Arch Microbiol 155: 348–352Google Scholar
  20. Thauer RK, Jungermann K, Dekker K (1977) Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev 41: 100–180Google Scholar
  21. Tschech A, Fuchs G (1989) Anaerobic degradation of phenol via carboxylation to 4-hydroxybenzoate: in vitro study of isotope exchange between 14CO2 and 4-hydroxybenzoate. Arch Microbiol 152: 594–599Google Scholar
  22. Widdel F (1992) The genus Desulfotomaculum. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH (eds) The prokaryotes, 2nd edn, vol. 1. Springer, New York Berlin Heidelberg, pp 1792–1799Google Scholar
  23. Widdel F, Pfennig N (1981) Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. I. isolation of new sulfate-reducing bacteria enriched with acetate from saline environments. Description of Desulfobacter postgatei gen. nov., sp. nov. Arch Microbiol 129: 395–400Google Scholar
  24. Widdel F, Pfennig N (1984) Dissimilatory sulfate-or sulfur-reducing bacteria. In: Krieg NR, Holt JG (eds) Bergey's manual of systematic bacteriology, vol 1. Williams and Wilkins, Baltimore London, pp 663–679Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Jan Kuever
    • 1
  • Juergen Kulmer
    • 1
  • Sigrid Jannsen
    • 1
  • Ulrich Fischer
    • 2
  • Karl-Heinz Blotevogel
    • 1
  1. 1.AG MikrobiologicUniversität OldenburgOldenburgGermany
  2. 2.AG GeomikrobiologieUniversität OldenburgOldenburgGermany

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