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Archives of Microbiology

, Volume 159, Issue 3, pp 282–288 | Cite as

Isolation and characterization of a new spore-forming sulfate-reducing bacterium growing by complete oxidation of catechol

  • Jan Kuever
  • Juergen Kulmer
  • Sigrid Jannsen
  • Ulrich Fischer
  • Karl-Heinz Blotevogel
Original Papers

Abstract

A new mesophilic sulfate-reducing bacterium, strain Groll, was isolated from a benzoate enrichment culture inoculated with black mud from a freshwater ditch. The isolate was a spore-forming, rod-shaped, motile, gram-positive bacterium. This isolate was able of complete oxidation of several aromatic compounds including phenol, catechol, benzoate, p-and m-cresol, benzyl alcohol and vanillate. With hydrogen and carbon dioxide, formate or O-methylated aromatic compounds, autotrophic growth during sulfate reduction or homoacetogenesis was demonstrated. Lactate was not used as a substrate. SO inf4 sup2- , SO inf3 sup2- , and S2O inf3 sup2- were utilized as electron acceptors. Although strain Groll originated from a freshwater habitat, salt concentrations of up to 30 g·l-1 were tolerated. The optimum temperature for growth was 35–37°C. The G+C content of DNA was 42.1 mol%. This isolate is described as a new species of the genus Desulfotomaculum.

Key words

Sulfate reduction Desulfotomaculum Anaerobic catechol oxidation Degradation and transformation of aromatic compounds 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Jan Kuever
    • 1
  • Juergen Kulmer
    • 1
  • Sigrid Jannsen
    • 1
  • Ulrich Fischer
    • 2
  • Karl-Heinz Blotevogel
    • 1
  1. 1.AG MikrobiologicUniversität OldenburgOldenburgGermany
  2. 2.AG GeomikrobiologieUniversität OldenburgOldenburgGermany

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