ATP synthesis from 2,3-diphosphoglycerate by cell-free extract of Methanobacterium thermoautotrophicum (strain ΔH)
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Cell-free extracts of Methanobacterium thermoautotrophicum were found to catalyze ATP synthesis from an endogeneous substrate. Synthesis was stimulated under hydrogen atmosphere and inhibited by KCL (Ki=150 mM). Comparison of the properties of a number of cell constituents showed the endogeneous substrate to be 2,3-diphosphoglycerate. The compound is converted into 3-phosphoglycerate, and via 2-phosphoglycerate and phosphoenolpyruvate into pyruvate, at which the latter reaction is linked with ATP synthesis.
Key wordsMethanogenic bacteria Methanobacterium thermoautotrophicum ATP synthesis 2,3-Diphosphoglycerate 2,3-Diphosphoglycerate phosphatase Cyclic 2,3-diphosphoglycerate
Coenzyme M, 2-mercaptoethanesulfonate
methylcoenzyme m, 2-(methylthio)ethanesulfonate
the heterodisulfide of HS-CoM and HS-HTP
bolled cell-free extract
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