D-Gluconate is an alternative growth substrate for cultivation of Schizosaccharomyces pombe mutants
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Schizosaccharomyces pombe cells grow on d-gluconate as the sole carbon and energy source. d-Gluconate is taken up in symport with protons by a specific symporter, ΔpH being the sole driving force. d-Gluconate uptake is independent of the sugar transporting system (e.g. for d-glucose) and of Δψ. The carrier is expressed constitutively, and its activity is not subject to glucose repression. Hence, d-gluconate is a suitable carbon and energy source for growth, when d-glucose or other hexoses have to be eliminated e.g. for selection of mutants deficient in hexose transport.
Key wordsd-Gluconate uptake H+-Symport Alternative growth substrate Schizosaccharomyces pombe
electrical potential difference across the plasma membrane
standard error of the mean
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