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Archives of Microbiology

, Volume 157, Issue 2, pp 176–182 | Cite as

Effect of coculture of anaerobic fungi isolated from ruminants and non-ruminants with methanogenic bacteria on cellulolytic and xylanolytic enzyme activities

  • M. J. Teunissen
  • E. P. W. Kets
  • H. J. M. Op den Camp
  • J. H. J. Huis in't Veld
  • G. D. Vogels
Original Papers
  • 223 Downloads

Abstract

Neocallimastix strain N1, an isolate from a ruminant (sheep), was cocultured with three Methanobacterium formicicum strains, Methanosarcina barkeri, and Methanobrevibacter smithii. The coculture with Methanobacterium formicicum strains resulted in the highest production of cellulolytic and xylanolytic enzymes. Subsequently four anaerobic fungi, two Neocallimastix strains (N1 and N2) from a ruminant and two Piromyces species from non-ruminants (E2 and R1), were grown in coculture with Methanobacterium formicicum DSM 3637 on filter paper cellulose and monitored over a 7-day period for substrate utilisation, fermentation products, and secretion of cellulolytic and xylanolytic enzymes. Methanogens caused a shift in fermentation products to more acetate and less ethanol, lactate and succinate. Furthermore the cellulose digestion rate increased by coculture. For cocultures of Neoallimastix strains with Methanobacterium formicicum strains the cellulolytic and xylanolytic enzyme production increased. Avicelase, CMCase and xylanase were almost completely secreted into the medium, while 40–60% of the β-glucosidase was found to be cell bound. Coculture had no significant effect on the location of cellulolytic and xylanolytic enzymes.

Key words

Anaerobic fungi Methanogenic bacteria Coculture Neocallimastix Cellulase secretion Enzyme location 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • M. J. Teunissen
    • 1
  • E. P. W. Kets
    • 1
  • H. J. M. Op den Camp
    • 1
  • J. H. J. Huis in't Veld
    • 2
  • G. D. Vogels
    • 1
  1. 1.Department of Microbiology, Faculty of ScienceUniversity of Nijmegen ToernooiveldNijmegenThe Netherlands
  2. 2.Department of BiotechnologyTNO Food and Nutrition ResearchZeistThe Netherlands

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