Archives of Microbiology

, Volume 157, Issue 2, pp 155–160 | Cite as

Autotrophic growth and inorganic sulphur compound oxidation by Sulfolobus sp. in chemostat culture

  • A. Nixon
  • P. R. Norris
Original Papers


Sulfolobus strain LM was grown in tetrathionate and thiosulphate-limited continuous culture. CO2 limitation resulted in a decrease of the steady-state biomass and an increase in the specific rate of thiosulphate oxidation so that substrate did not accumulate in the medium. The initial step in thiosulphate utilization appeared to be its conversion to tetrathionate. The affinity for tetrathionate oxidation appeared to increase with prolonged continuous culture giving an apparent Km of about 6 μM tetrathionate, a higher affinity than for thiosulphate oxidation and in the same range as values observed with acidophilic, sulphur-oxidizing eubacteria.

Key words

Sulfolobus Chemostat culture Thiosulphate oxidation Tetrathionate oxidation 


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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • A. Nixon
    • 1
  • P. R. Norris
    • 1
  1. 1.Department of Biological SciencesUniversity of WarwickCoventryUK

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