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Archives of Microbiology

, Volume 157, Issue 2, pp 135–140 | Cite as

Phosphate-limited growth of Chromatium vinosum in continuous culture

  • Jordi Mas
  • Hans van Gemerden
Original Papers

Abstract

Chromatium vinosum DSM 185 was grown in continuous culture at a constant dilution rate of 0.071 h-1 with sulfide as the only electron donor. The organism was subjected to conditions ranging from phosphate limitation (SR-phosphate=2.7 μM and SR-sulfide=1.8 mM) to sulfide limitation (SR-phosphate=86 μM and SR-sulfide=1.8 mM). At values of SR-phosphate below 7.5 μM the culture was washed out, whereas SR-phosphate above this value resulted in steady states. The saturation constant (Kμ) for growth on phosphate was estimated to be between 2.6 and 4.1 μM. The specific phosphorus content of the cells increased from 0.30 to 0.85 μmol P mg-1 protein with increasing SR-phosphate. The specific rate of phosphate uptake increased with increasing SR-phosphate, and displayed a non-hyperbolic saturation relationship with respect to the concentration of phosphate in the inflowing medium. Approximation of a hyperbolic saturation function yielded a maximum uptake rate (Vmax) of 85 nmol P mg-1 protein h-1, and a saturation constant for uptake (Kt) of 0.7 μM. When phosphate was supplied in excess 8.5% of the phosphate taken up by the cells was excreted as organic phosphorus at a specific rate of 8 nmol P mg-1 protein h-1.

Key words

Chromatium vinosum Phosphate Nutrient limitation Continuous culture Phototrophic bacteria 

Non-standard abbreviations

BChla

bacteriochlorophyll a

D

dilution rate; μmax, maximum specific growth rate

μ

maximum specific growth rate if the substrate were not inhibitory

Kμ

saturation constant for growth on phosphate

Vmax

maximum rate of phosphate uptake

Ki

saturation constant for phosphate uptake

Ki

inhibition constant for growth in the presence of sulfide

SR

concentration of substrate in the inflowing medium

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Jordi Mas
    • 1
  • Hans van Gemerden
    • 1
  1. 1.Department of MicrobiologyUniversity of GroningenHarenThe Netherlands

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