Advertisement

Archives of Microbiology

, Volume 154, Issue 5, pp 489–495 | Cite as

Catabolism of 3-hydroxybenzoate by the gentisate pathway in Klebsiella pneumoniae M5a1

  • David C. N. Jones
  • Ronald A. Cooper
Original Papers

Abstract

Growth of Klebsiella pneumoniae M5a1 on 3-hydroxybenzoate leads to the induction of 3-hydroxybenzoate monooxygenase, 2,5-dihydroxybenzoate dioxygenase, maleylpyruvate isomerase and fumarylpyruvate hydrolase. Growth in the presence of 2,5-dihydroxybenzoate also induces all of these enzymes including the 3-hydroxybenzoate monooxygenase which is not required for 2,5-dihydroxybenzoate catabolism. Mutants defective in 3-hydroxybenzoate monooxygenase fail to grow on 3-hydroxybenzoate but grow normally on 2,5-dihydroxybenzoate. Mutants lacking maleylpyruvate isomerase fail to grow on 3-hydroxybenzoate and 2,5-dihydroxybenzoate. Both kinds of mutants grow normally on 3,4-dihydroxybenzoate. Mutants defective in maleylpyruvate isomerase accumulate maleylpyruvate when exposed to 3-hydroxybenzoate and growth is inhibited. Secondary mutants that have additionally lost 3-hydroxybenzoate monooxygenase are no longer inhibited by the presence of 3-hydroxybenzoate. The 3-hydroxybenzoate monooxygenase gene (mhbM) and the maleylpyruvate isomerase gene (mhbI) are 100% co-transducible by P1 phage.

Key words

Klebsiella pneumoniae M5a1 3-Hydroxybenzoate degradation Gentisate pathway 3-Hydroxybenzoate monooxygenase mutants Maleylpyruvate isomerase mutants 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bayly RC, Chapman PJ, Dagley S, DiBerardino D (1980) Purification and some properties of maleylpyruvate hydrolase and fumarylpyruvate hydrolase from Pseudomonas alcaligenes. J Bacteriol 143: 70–77PubMedPubMedCentralGoogle Scholar
  2. Burlinghame R, Chapman PJ (1983) Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli. J Bacteriol 155: 113–121Google Scholar
  3. Cooper RA, Skinner MA (1980) Catabolism of 3- and 4-hydroxyphenylacetate by the 3,4-dihydroxyphenylacetate pathway in Escherichia coli. J Bacteriol 143: 302–306PubMedPubMedCentralGoogle Scholar
  4. Cooper RA, Jones DCN, Parrott S (1985) Isolation and mapping of Escherichia coli K12 mutants defective in phenylacetate degradation. J Gen Microbiol 131: 2753–2757PubMedGoogle Scholar
  5. Czok R, Lamprecht W (1974) Pyruvate, phosphoenolpyruvate and d-glycerate 2-phosphate. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol 3, 2nd english edn. Verlag Chemie, Weinheim; Academic Press, New York London, pp 1446–1451Google Scholar
  6. Dagley S (1975) A biochemical approach to some problems of environmental pollution. In: Campbell PN, Aldridge WN (eds) Essays in biochemistry, vol 11. Academic Press, London New York San Francisco, pp 81–138Google Scholar
  7. Dagley S, Chapman PJ, Gibson DT (1965) The metabolism of β-phenylpropionic acid by an Achromobacter. Biochem J 97: 643–650CrossRefGoogle Scholar
  8. Deschamps AM, Richard C, Lebeault J-M (1983) Bacteriology and nutrition of environmental strains of Klebsiella pneumoniae involved in wood and bark decay. Ann Microbiol (Paris) 134A: 189–196Google Scholar
  9. Dixon RA (1984) The genetic complexity of nitrogen fixation. J Gen Microbiol 130: 2745–2755PubMedGoogle Scholar
  10. Doten RC, Ornston LN (1987) Protocatechuate is not metabolized via catechol in Enterobacter aerogenes. J Bacteriol 169: 5827–5830CrossRefGoogle Scholar
  11. Gornall AC, Bardawill CS, David MM (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177: 751–766PubMedGoogle Scholar
  12. Grant DJW, Patel JC (1969) The non-oxidative decarboxylation of p-hydroxybenzoic acid, gentisic acid, protocatechuic acid and gallic acid by Klebsiella aerogenes (Aerobacter aerogenes). Antonie van Leuwenhoek 35: 325–343CrossRefGoogle Scholar
  13. Groseclose EE, Ribbons DW (1973) 3-Hydroxybenzoate 6-hydroxylase from Pseudomonas aeruginosa. Biochem Biophys Res Commun 55: 897–903CrossRefGoogle Scholar
  14. Gutmann I, Wahlefeld AW (1976) l−(-)-Malate. Determination with malate dehydrogenase and NAD. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol 3, 2nd english edn. Verlag Chemie, Weinheim; Academic Press, New York London, pp 1585–1589Google Scholar
  15. Hareland WA, Crawford RL, Chapman PJ, Dagley S (1975) Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans. J Bacteriol 121: 272–285PubMedPubMedCentralGoogle Scholar
  16. Jenkins JR, Cooper RA (1988) Molecular cloning, expression, and analysis of the genes of the homoprotocatechuate catabolic pathway of Escherichia coli C. J Bacteriol 170: 5317–5324CrossRefGoogle Scholar
  17. Lack L (1959) The enzymatic oxidation of gentisic acid. Biochim Biophys Acta 34: 117–123CrossRefGoogle Scholar
  18. Lack L (1961) Enzymatic cis-trans isomerization of maleylpyruvate. J Biol Chem 236: 2835–2840PubMedGoogle Scholar
  19. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USAGoogle Scholar
  20. Nordmann J, Nordmann R (1969) Organic acids. In: Smith I (ed) Chromatographic and electrophoretic techniques, 3rd edn. William Heinemann, London, pp 342–363Google Scholar
  21. Parrott S, Jones S, Cooper RA (1987) 2-Phenylethylamine catabolism by Escherichia coli K12. J Gen Microbiol 133: 347–351PubMedGoogle Scholar
  22. Patel JC, Grant DJW (1969) The formation of phenol in the degradation of p-hydroxybenzoic acid by Klebsiella aerogenes (Aerobacter aerogenes). Antonie van Leuwenhoek 35: 53–64CrossRefGoogle Scholar
  23. Skinner MA, Cooper RA (1982) An Escherichia coli mutant defective in the NAD-dependent succinate semialdehyde dehydrogenase. Arch Microbiol 132: 270–275CrossRefGoogle Scholar
  24. Smith I, Seakins JWT, Dayman J (1969) Phenolic acids. In: Smith I (ed) Chromatographic and electrophoretic techniques, 3rd edn. William Heinemann, London, pp 364–389Google Scholar
  25. Sparnins VL, Chapman PJ, Dagley S (1974) Bacterial degradation of 4-hydroxyphenylacetic acid and homoprotocatechuic acid. J Bacteriol 120: 159–167PubMedPubMedCentralGoogle Scholar
  26. Streicher S, Gurney E, Valentine RC (1971) Transduction of the nitrogen-fixation genes in Klebsiella pneumoniae. Proc Natl Acad Sci USA 68: 1174–1177CrossRefGoogle Scholar
  27. Wheelis ML, Palleroni NJ, Stanier RY (1967) The metabolism of aromatic compounds by Pseudomonas testosteroni and P. acidovorans. Arch Mikrobiol 59: 302–314CrossRefGoogle Scholar
  28. Yano K, Arima K (1958) Metabolism of aromatic compounds by bacteria, II. m-Hydroxybenzoic acid hydroxylase A and B; 5-dehydroshikimic acid, a precursor of protocatechuic acid, a new pathway from salicylic acid to gentisic acid. J Gen Appl Microbiol 4: 241–258CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • David C. N. Jones
    • 1
  • Ronald A. Cooper
    • 1
  1. 1.Department of BiochemistryUniversity of LeicesterLeicesterUK

Personalised recommendations