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Archives of Microbiology

, Volume 154, Issue 5, pp 483–488 | Cite as

Cell wall anionic polymers and peptidoglycan of Actinoplanes philippinensis VKM Ac-647

  • Irina B. Naumova
  • Nailya A. Yanushkene
  • Galina M. Streshinskaya
  • Alexander S. Shashkov
Original Papers

Abstract

The cell wall of Actinoplanes philippinesis VKM Ac-647 harbours several carbohydrate-containing anionic polymers. (1) The main polymer of the wall is of a poly(glycosylglycerol phosphate) nature. Its monomeric units — O-α-d-mannopyranosyl-(1→4)-β-d-galactopyranosyl-(1→1)-glycerol monophosphates — are connected by phosphodiester bonds involving the hydroxyl groups at glycerol C3 and galactose C6. There also are chains without mannosyl substitutents. The teichoic acid structure has been established by chemical analysis and with 1H and 13C NMR spectroscopy. This is the first finding of a teichoic acid with mannosyl residues in a bacterial cell wall. (2) The phosphorylated mannan contains mannose and 2-O-methylmannose. Its core chain has α-1,2; α-1,3; and α-1,6 substitutions as revealed by 13C NMR spectroscopy.

The peptide unit of the peptidoglycan contains no l-alanine, instead of which position 1 is occupied by glycine; and diaminopimelic acid is represented, besides its meso- (or DD) form, by small amounts of its LL isomer.

Key words

Actinoplanes Teichoic acid 2-O-methylmannose Peptidoglycan 

Abbreviations

Gro

glycerol

Gro2P

glycerol-2 phosphate

APT

attached-proton-test

Ptot

total content of phosphorus

Plab

phosphorus mineralized in 7 min at 100°C

PNA

phosphorus of nucleic acids

Pstab

stable phosphorus

T

trace amounts

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Irina B. Naumova
    • 1
  • Nailya A. Yanushkene
    • 1
  • Galina M. Streshinskaya
    • 1
  • Alexander S. Shashkov
    • 2
  1. 1.Department of BiologyMoscow State UniversityMoscowUSSR
  2. 2.N. D. Zelinsky Institute of Organic ChemistryUSSR Academy of SciencesMoscowUSSR

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