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Archives of Microbiology

, Volume 154, Issue 5, pp 465–470 | Cite as

Microbial degradation of the morphine alkaloids: identification of morphinone as an intermediate in the metabolism of morphine by Pseudomonas putida M10

  • Neil C. Bruce
  • Clare J. Wilmot
  • Keith N. Jordan
  • Anna E. Trebilcock
  • Lauren D. Gray Stephens
  • Christopher R. Lowe
Original Papers

Abstract

A strain of Pseudomonas putida was isolated by selective enrichment with morphine that was capable of utilising morphine as a primary source of carbon and energy for growth. Experiments with whole cells showed that both morphine and codeine, but not thebaine, could be utilised. A novel NADP-dependent dehydrogenase, morphine dehydrogenase, was purified from crude cell extracts and was shown to be capable of oxidising morphine and codeine to morphinone and codeinone, respectively. This NADP-dependent morphine dehydrogenase was not observed in any other species of pseudomonads examined and was quite distinct from the β-hydroxysteroid dehydrogenase found in Pseudomonas testosteroni, which had previously been shown to have activity against morphine.

Key words

Pseudomnas putida Morphine Codeine Codeinone Morphinone Alkaloids Morphine dehydrogenase 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Neil C. Bruce
    • 1
  • Clare J. Wilmot
    • 1
  • Keith N. Jordan
    • 1
  • Anna E. Trebilcock
    • 1
  • Lauren D. Gray Stephens
    • 1
  • Christopher R. Lowe
    • 1
  1. 1.Institute of BiochnologyUniversity of CambridgeCambridgeUK

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