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Archives of Microbiology

, Volume 154, Issue 5, pp 438–442 | Cite as

Polyphosphate glucokinase and ATP glucokinase activities in Mycococcus coralloides D

  • Francisco González
  • Antonia Fernández-Vivas
  • José M. Arias
  • Enrique Montoya
Original Papers

Abstract

Polyphosphate glucokinase and ATP glucokinase were detected in cell-free extracts of Myxococcus coralloides strain D, but pyrophosphate glucokinase was not detected. Both glucokinase activities were separated by chromatography. The approximate molecular weight is 61 000 for polyphosphate glucokinase and 47 000 for ATP glucokinase. Substrate specificity and pH optimum was studied in the polyphosphate glucokinase. Polyphosphate and ATP glucokinase activities were verified by 13C Nuclear Magnetic Resonance.

Key words

13C-NMR Glucokinase Polyphosphate Myxococcus 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Francisco González
    • 1
  • Antonia Fernández-Vivas
    • 1
  • José M. Arias
    • 1
  • Enrique Montoya
    • 1
  1. 1.Departmento de Microbiología, Facultad de CienciasUniversidad de GranadaGranadaSpain

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