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Archives of Microbiology

, Volume 154, Issue 5, pp 433–437 | Cite as

The lipopolysaccharides of Rhodospirillum rubrum, Rhodospirillum molischianum, and Rhodopila globiformis

  • Klaus Pietsch
  • Jürgen Weckesser
  • Ulrich Fischer
  • Hubert Mayer
Original Papers

Abstract

The cell wall lipopolysaccharides from three phototrophic species of the alpha1-group of Proteobacteria, Rhodospirillum rubrum, Rhodospirillum molischianum, and Rhodopila globiformis were isolated and chemically characterized. Sodium deoxycholate polyacrylamide gel electrophoresis patterns revealed that the lipopolysaccharides of all three species possess O-chains. They are composed of repeating units only in R. molischianum and R. globiformis. The presence of l-glycero-d-mannoheptose and 2-keto-3-deoxyoctonate indicated core structures in all three lipopolysaccharides. Glucosamine was found as backbone amino sugar in lipid A of R. molischianum and R. rubrum, while R. globiformis has 2,3-diaminoglucose as backbone amino sugar. The latter species also differed from the two former ones in its content of hydroxy fatty acids (3-OH-14:0, 3-OH-16:0 in R. rubrum and R. molischianum and 3-OH-14:0, 3-OH-18:0 and 3-OH-19:0 (possibly iso- or anteisobranched) in R. globiformis).

Key words

Rhodospirillum rubrum Rhodospirillum molischianum Rhodopila globiformis Lipopolysaccharide Lipid A 2,3-Diamino-glucose 

Abbreviations

DOC-PAGE

sodium deoxycholate polyacrylamide gel electrophoresis

GC/MS

combined gas-liquid chromatography/mass spectrometry

KDO

2-keto-3-deoxyoctonate

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Klaus Pietsch
    • 1
  • Jürgen Weckesser
    • 2
  • Ulrich Fischer
    • 3
  • Hubert Mayer
    • 1
  1. 1.Max-Planck-Institut für ImmunbiologieFreiburg i. Br.Germany
  2. 2.Institut für Biologie II, MikrobiologieAlbert-Ludwigs-UniversitätFreiburg i. Br.Germany
  3. 3.Fachbereich Biologie, AG GeomikrobiologieUniversität OldenburgOldenburgGermany

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