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Archives of Microbiology

, Volume 154, Issue 5, pp 417–421 | Cite as

Lithoautotrophic growth of the iron bacterium Gallionella ferruginea with thiosulfate or sulfide as energy source

  • Susanne Lütters-Czekalla
Original Papers

Abstract

Gallionella ferruginea is able to utilize Fe(II) and the reduced sulfur compounds sulfide and thiosulfate as electron donor and energy source. Tetrathionate and elemental sulfur, on the other hand, are not metabolized. In sulfide-O2 microgradient cultures G. ferruginea grows at the interface between the oxidizing and the reducing zones. Optimal growth depends on low oxygen and sulfide concentrations. Establishing within the gradient protects the bacterium from too high sulfide concentrations. G. ferruginea excretes extracellular polymeric substances (EPS). While in FeS-gradient cultures 2×106 cells/ml were obtained the bacterial mass could be increased to 1−3×108 cells/ml in shaken batch cultures using thiosulfate as substrate. A further increase of bacterial mass by adding an organic carbon source was not possible confirming that G. ferruginea is an obligate autotrophic organism. When growing on sulfide or thiosulfate the otherwise characteristic twisted stalk consisting of ferric hydroxide is lacking. It is thus shown to be a metabolic end product of Fe(II) oxidation rather than metabolically active cellular material.

Key words

Gallionella ferruginea Thiobacillus ferrooxidans Iron bacteria Chemolithoautotroph Thiosulfate oxidation Sulfide oxidation Sulfide gradient culture Microaerobe Stalk production Extracellular polymeric substances 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Susanne Lütters-Czekalla
    • 1
  1. 1.Institut für Mikrobiologie der Technischen Universität BraunschweigBraunschweigGermany

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