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Applied Microbiology and Biotechnology

, Volume 38, Issue 4, pp 507–513 | Cite as

Characterization, cloning and sequencing of a thermostable endo-(1,3–1,4) β-glucanase-encoding gene from an alkalophilic Bacillus brevis

  • Maureen E. Louw
  • Sharon J. Reid
  • T. G. Watson
Applied Genetics and Regulation

Abstract

A Bacillus brevis gene coding for an endo-(1,3–1,4)-β-glucanase was cloned in Escherichia coli and sequenced. The open reading frame contains a sequence of 759 nucleotides encoding a polypeptide of 252 amino acid residues. The amino acid sequence of the β-glucanase gene showed only a 50% similarity to previously published data for Bacillus endo-(1,3–1,4)-β-glucanases. The optimum temperature and pH for enzyme activity were 65–70°C and 8–10, respectively. When held at 75°C for 1 h, 75% residual activity was measured. The molecular mass was estimated to be about 29 kDa on sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis and the enzyme was found to be resistant to SDS.

Keywords

Enzyme Escherichia Coli Nucleotide Amino Acid Sequence Electrophoresis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Maureen E. Louw
    • 1
  • Sharon J. Reid
    • 1
  • T. G. Watson
    • 2
  1. 1.Biotechnology Programme, Division of Food Science and TechnologyCSIRPretoriaRepublic of South Africa
  2. 2.Department of MicrobiologyUniversity of Cape Town, Private BagCape TownRepublic of South Africa

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