Applied Microbiology and Biotechnology

, Volume 38, Issue 4, pp 507–513 | Cite as

Characterization, cloning and sequencing of a thermostable endo-(1,3–1,4) β-glucanase-encoding gene from an alkalophilic Bacillus brevis

  • Maureen E. Louw
  • Sharon J. Reid
  • T. G. Watson
Applied Genetics and Regulation


A Bacillus brevis gene coding for an endo-(1,3–1,4)-β-glucanase was cloned in Escherichia coli and sequenced. The open reading frame contains a sequence of 759 nucleotides encoding a polypeptide of 252 amino acid residues. The amino acid sequence of the β-glucanase gene showed only a 50% similarity to previously published data for Bacillus endo-(1,3–1,4)-β-glucanases. The optimum temperature and pH for enzyme activity were 65–70°C and 8–10, respectively. When held at 75°C for 1 h, 75% residual activity was measured. The molecular mass was estimated to be about 29 kDa on sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis and the enzyme was found to be resistant to SDS.


Enzyme Escherichia Coli Nucleotide Amino Acid Sequence Electrophoresis 
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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Maureen E. Louw
    • 1
  • Sharon J. Reid
    • 1
  • T. G. Watson
    • 2
  1. 1.Biotechnology Programme, Division of Food Science and TechnologyCSIRPretoriaRepublic of South Africa
  2. 2.Department of MicrobiologyUniversity of Cape Town, Private BagCape TownRepublic of South Africa

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