Applied Microbiology and Biotechnology

, Volume 38, Issue 4, pp 453–457 | Cite as

Fermentation of glycerol to 1,3-propanediol in continuous cultures of Citrobacter freundii

  • Rainer Boenigk
  • Susanne Bowien
  • Gerhard Gottschalk


The conversion of glycerol to 1,3-propanediol by Citrobacter freundii DSM 30040 was optimized in single- and two-stage continuous cultures. The productivity of 1,3-propanediol formation was highest under glycerol limitation and increased with the dilution rate (D) to a maximum of 3.7 g·l−1·h−1. Glycerol dehydratase seemed to be the rate-limiting step in 1,3-propanediol formation. Conditions for the two-stage fermentation process were as follows: first stage, glycerol limitation (250mM), pH 7.2, D=0.1 h, 31° C; second stage, additional glycerol, pH 6.6, D=0.05 h−1, 28° C. Under these conditions 875mM glycerol were consumed, the final 1,3-propanediol concentration was 545mM, and the overall productivity 1.38 g·1−1·h−1.


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Rainer Boenigk
    • 1
  • Susanne Bowien
    • 1
  • Gerhard Gottschalk
    • 1
  1. 1.Institut für MikrobiologieGeorg-August-Universität GöttingenGöttingenFederal Republic of Germany

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