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Biotechnology Techniques

, Volume 8, Issue 9, pp 639–644 | Cite as

Inoculation and tissue distribution in pilot-scale plant root culture bioreactors

  • Divakar Ramakrishnan
  • John Salim
  • Wayne R. Curtis
Article

Summary

Inoculation of large-scale plant root culture reactors can be carried out by briefly homogenizing bulk root tissue, followed by aseptic transfer as a slurry to the reactor. Uniform root distribution can be achieved in bioreactors by entrapment of the growing root inoculum onto process packing elements (e.g. distillation packings), randomly distributed within the reactor, in a bubble column operation. These two techniques have been successfully used to inoculate a 14 L pilot-scale reactor which is subsequently operated as a trickle bed reactor.

Keywords

Tissue Distribution Root Tissue Root Culture Process Packing Root Distribution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • Divakar Ramakrishnan
    • 1
  • John Salim
    • 1
  • Wayne R. Curtis
    • 1
    • 2
    • 3
  1. 1.Department of Chemical EngineeringThe Pennsylvania State UniversityUniversity ParkUSA
  2. 2.The Biotechnology Institute The Pennsylvania State UniversityUniversity ParkUSA
  3. 3.111 Fenske Laboratory, Dept. of Chemical EngineeringPSUUniversity ParkUSA

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